Novel helper plasmid, defective sindbis viral vectors and methods of use thereof

ABSTRACT

Disclosed herein are new defective Sindbis viral vectors made from a novel Helper plasmid, with differences in envelope proteins between JT vectors and consensus Sindbis virus sequences, and also between JT and Ar-339 vectors. Also disclosed are vectors produced using the plasmid, methods for producing the vectors, methods for treating mammals suffering from tumors and pharmaceutical formulations for use in the treatment methods.

CROSS REFERENCE TO RELATED APPLICATIONS

The present application claims the benefit of U.S. Provisional Patent Application Ser. No. 61/061,493, filed on Jun. 13, 2008, and U.S. Provisional Patent Application Ser. No. 61/092,343, filed on Aug. 27, 2008, both of which are hereby incorporated by reference in their entireties.

STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT

The United States Government has certain rights to this invention by virtue of funding received from Group CA 100681 from the National Institutes of Health, Department of Health and Human Services.

FIELD OF THE INVENTION

The present invention is directed to defective Sindbis viral vectors, plasmids used to produce such vectors, pharmaceutical formulations containing the vectors, and methods for their production and use to treat mammals suffering from tumors.

BACKGROUND OF THE INVENTION

Sindbis virus, a member of the alphavirus genus in the Togaviridae family, is a single-stranded, enveloped, positive-sense RNA virus (Strauss & Strauss, 1994). In nature, it is transmitted via mosquito bites to mammals. Thus, as Sindbis virus has evolved as a blood-borne vector, this hematogenous delivery property enables Sindbis vectors to reach tumor cells throughout the circulation (Tseng et al 2004a,b).

U.S. Pat. No. 7,306,792, which is the National Stage of PCT/US02/09432 published as WO 02/076468 entitled TUMOR THERAPY WITH ALPHAVIRUS-BASED AND HIGH AFFINITY LAMININ RECEPTOR-TARGETED VECTORS discloses methods for treating solid tumors in mammals using Sindbis vectors. The method comprises systemically administering to a mammal harboring a tumor an amount of an Alphavirus vector effective to treat the tumor. The vector was said to have a preferential affinity for high affinity laminin receptors (HALR). Tumor cells which express greater levels of HALR compared to normal cells of the same lineage were preferentially killed by the vector. The anti-tumor effect was said to be due to the fact that Sindbis virus infection induced apoptosis in infected cells.

PCT/US 2004/026671 for A METHOD FOR DETECTING CANCER CELLS AND MONITORING CANCER THERAPY discloses the use of Sindbis viral vectors to identify cancer cells in the body of a mammal and monitor anti-cancer therapy.

U.S. Pat. No. 7,303,898 discloses defective Sindbis virus vectors, novel Helper and Replicon plasmids used to produce the vectors and methods for treating mammals harboring solid tumors which express higher levels of HALR than normal cells of the same lineage.

U.S. Pat. No. 7,378,272 discloses packaging cell lines for the continuous production of defective Sindbis virus vectors.

With the aim of broadening the knowledge of the way Sindbis vectors work for cancer gene therapy, two different kinds of Sindbis vectors, SP6-H/SP6-R, derived from wild type Ar-339, and JT-BB/JT-Rep derived from an Ar-339 laboratory adapted strain, Toto 1101 have been studied. Sindbis virus Ar-339 was first isolated in August 1952, from a pool of mosquitoes (Culex pipiens and C. univittatus) trapped in the Sindbis health district in Egypt (Hurlbut 1953; Taylor and Hurlbut 1953; Frothingham 1955; Taylor et al. 1955). Toto 1101 was made out of the heat resistant (HR) strain initially derived from AR-339 (Burge and Pfefferkorn, 1966). The first studies done with JT vectors in animal models showed good targeting of tumor cells and significant reduction of metastatic implant size (Tseng et al. 2002). Further studies of these vectors in tumor-induced SCID mice were done using the new imaging technique of IVIS®, that allows in vivo detection of viral vector and tumor cells in the same animal. In tumor-induced SCID mice there was a good correlation between vectors and tumor cells (Tseng et al. 2004b). Although these positive results in vector targeting and in vivo growth reduction of tumors and mouse survival, which are very promising for gene therapy, survival of all mice in these tumor models has not yet been achieved.

Therefore, what is need in the art are improved Sindbis viral vectors for use as anti-tumor agents.

BRIEF SUMMARY OF THE INVENTION

Disclosed herein are new vectors made from wild type Ar-339 Sindbis virus, with differences in replicase and envelope proteins between JT vectors and consensus Sindbis virus sequences, and also between JT and Ar-339 vectors. The chimeras combining both strains were produced and studied in tumor-induced SCID mice by the IVIS® imaging technique. Surprisingly JT envelope proteins targeted tumors more effectively than Ar-339 while Ar-339 replicase showed increased efficiency in tumor reduction. To analyze which residues would be responsible for tumor targeting, mutants of Ar-339 E2 envelope protein were made and tested by IVIS® imaging in ES-2 induced and tumor free mouse models. The change of only one amino acid from Glu to Lys at position 70 of Ar-339 E2, suppressed the ability to target metastatic tumor implants in mice. Double E2 mutant Mut-2, with K70 and V251 did not revert the targeting. Only when the whole sequence of JT E2 was substituted in the Ar-339 helper was the ability of targeting metastatic tumor implants recovered, though with less intensity. Thus, residue 70 in the outer leaf of the E2 protein is essential for tumor specific targeting of Sindbis vectors.

In one aspect, the present invention provides a purified, isolated nucleic acid comprising a nucleotide sequence encoding the DM Helper plasmid.

In yet a further aspect, the present invention provides a method for producing defective Sindbis viral vectors comprising the steps of

-   -   (a) providing a linearized replicon plasmid comprising the         nucleotide sequence as set forth in SEQ ID NO: 36 and a         linearized Helper plasmid comprising the nucleotide sequence         encoding the DM Helper plasmid.     -   (b) transcribing said replicon plasmid and said Helper plasmid         to produce RNA;     -   (c) collecting the RNA transcribed in step (b) and transfecting         cells with said RNA;     -   (d) incubating said transfected cells for a time and at a         temperature effective for producing defective Sindbis viral         vectors; and     -   (e) collecting said defective Sindbis viral vectors from the         medium of said transfected cells.

In a still further aspect, the present invention provides an isolated nucleic acid comprising the nucleotide sequence encoding plasmid T7 DM-H101.

In a still further aspect, the present invention provides an isolated nucleic acid comprising the nucleotide sequence encoding plasmid T7-StuI-RLacZ#202.

These and other aspects of the present invention will be apparent to those of ordinary skill in the art in light of the present specification, claims and figures.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 Sindbis virus Ar-339 cDNA cloning.

Ar-339 11703 nt genomic RNA, is illustrated schematically in grey line, viral subgenomic promoter (P_(SG)) nt 7334 to 7646, is represented as grey solid box. The viral genome was cloned in 6 PCR overlapping fragments. The viral replicase (grey dotted line) and the subgenomic promoter were cloned in 4 PCR fragments: CDNA-1, CDNA-2, CDNA-3A and CDNA-3B. The Sindbis subgenomic promoter and structural proteins sequence (gray dashed line) were cloned in 2 PCR overlapping fragments CDNA-4 and CDNA-5. The position of restriction enzyme sites in the PCR fragments that were used for further cloning strategy are indicated.

FIG. 2. Sindbis replicon and helper plasmids.

The Sindbis virus Ar-339 genome was split in two to generate both replicon and helper plasmids. Viral sequences are represented in grey. Virus nucleotide numbers are indicated and follow the Strauss et al. 1984 sequence.

FIG. 3. Summary of vector constructions.

The first step generated the plasmid T7-pUC-PolyA#114 that contains the bacterial segment (ampicillin resistance, pMB1 replication origin), bacteriophage promoter T7, the two multicloning sites (MCS1 and MCS2) and the 3′ end of the virus sequence (grey dashed line). To generate the replicon, restriction enzyme (RE) digested and gel purified DNA fragments from CDNA-1, CDNA-2 and CDNA-3 were cloned sequentially into T7-pUC-PolyA#114. To generate the helper, first the MfeI/BamHI fragment from the CDNA-1 plasmid was cloned into T7-pUC-PolyA#114, and bands from CDNA-4 and CDNA-5 were cloned into this plasmid. The sequences of the primers used to clone the Ar-339 cDNA fragments are shown in Table I (Appendix A).

FIGS. 4(A and B). Suppression of disease progression by Ar-339 and JT chimeric vectors. A) ES-2/Fluc cells (1.5×10⁶) were i.p. inoculated into SCID mice on day 0. The next day (day 1), mice were imaged using the IVIS® Imaging System using D-luciferin as the substrate and were split into five groups of five mice each: control, Which received no vector treatment, vector A (JT-BB/SP6-RhRLuc), vector B (SP6-H/JT-RephRluc), vector C (SP6-H/SP6-RhRluc) and vector D (JT-BB/JT-RephRluc). On day 5 the groups received daily i.p. treatments of corresponding Sindbis vectors and were IVIS® imaged on days 1, 5, 13 and 19. All vector treatments suppressed the tumor growth on the mesentery and diaphragm and reduced the signals on the omentum compared with control mice. B) Quantitative analysis of the whole-body total photon counts of control and Sindbis-treated mice. Error bars represent the SEM.

FIG. 5. Survival curves of mice treated with Ar-339 and JT vectors. Survival curve of mice described in FIG. 4. Vector A was the most efficient in prolonging the survival of mice bearing ES-2/Fluc tumors.

FIGS. 6 (A and B). Colocalization in peritoneal cavity of vector C. A) Vector C (SP6-H/SP6-RhRluc) infection colocalized with the metastasized ES-2/Fluc tumors in the peritoneal cavity as determined by the IVIS® system. SCID mice were i.p. inoculated with 1.5×10⁶ ES-2/Fluc cells. Five days later, while the disease was still microscopic, inoculated mice received a single i.p. treatment of Vector C and were imaged the next day. The first IVIS® imaging was done by i.p. injection of Rluc substrate, coelenterazine, followed by a 5-minute acquiring interval (left panel). Thirty minutes after the coelenterazine injection, when the short-lived Rluc signals faded away, Fluc substrate, D-luciferin, was i.p. injected to determine the ES-2/Fluc tumor locations (right panel). B) Correlation analysis of vector C shows a high correspondence between tumor cells and vector infection in the peritoneal cavity.

FIGS. 7(A and B). Background infection of Ar-339 and JT chimeric vectors. A) Five tumor-free mice per group were i.p. injected on day 0 with one dose of vector A (JT-BB/SP6-RFluc), vector B (SP6-H/JT-RepFluc) or vector C (SP6-H/SP6-RFluc) and next day (day 1) IVIS® imaged for vector luciferase signal). The peritoneum was removed for imaging of the peritoneal cavity and the organs were harvested and imaged for the representative mice (rows 3 to 5). All vectors showed infection in fat tissue, and in vector B and C groups some mice showed a low background signal on ribs but not in organs B) Some mice per group received a second i.p. injection of the vectors of day 2, and were IVIS® imaged in: the peritoneal cavities (second row) and organs (bottom rows) on day 3. Very low signals were observed in fat tissue for vectors B and C.

FIGS. (8A and B). Tumor targeting of Ar-339 and JT chimeric vectors. A) SCID female mice were injected i.p. on day 0 with 2×10⁶ ES-2 cells/mouse. On day 4 five mice per group were i.p. injected with one doses of vector A (JT-BB/SP6-RFluc), vector B (SP6-H/JT-RepFluc) or vector C (SP6-H/SP6-RFluc) and next day (day 5) IVIS® imaged for vector luciferase signals. The peritoneum was removed for imaging of the peritoneal cavity and the organs were harvested and imaged. All vectors targeted tumor implants. Tumors on the peritoneum, pancreas-omentum and bowel are circled. B) Some mice per group received a second i.p. injection of the vectors of day 6, and the peritoneal cavities (second row) and organs (bottom rows) were IVIS® imaged on day 7. One mouse per group (#28, 33, 38) was not injected to serve as a luciferase background control. Vectors B and C showed decreased bioluminescence signals in tumors compared with the first injection (A).

FIG. 9A-9E. SP6-H Ar-339 E2 mutants. Amino acids changed in Ar-339 E2 mutants Mut-1 (9C), Mut-2 (9D) and Mut-4 (9E). Sequences and residues from the JT-BB plasmid are shown in 9A, those corresponding to Ar-339 are represented in 9B.

FIGS. 10(A and B). Background infection of Ar-339 E2 mutants. A) Five tumor-free mice per group were i.p. injected on day 0 with one dose of vector A (JT-BB/SP6-RFluc), vector C (SP6-H/SP6-RFluc), Mut-1(SP6-H-K70/SP6-RFluc), Mut-2(SP6-H-K70-V251/SP6-RFluc) and Mut-4(SP6-H-I3-K70-E181-V251/SP6-RFluc). The next day, mice were IVIS® imaged for vector luciferase signals. The peritoneum was removed for imaging of the peritoneal cavity of representative mice and the organs were harvested and also imaged. The E2 mutant vectors did not show background infection of fat tissue as observed with vectors A and C. E2 mutant organ arrays were also IVIS® imaged at Bin 10 resolution to increase the detection limit (bottom row), arrows point to regions with signals. B) Some mice per group received a second i.p. injection of the vectors of day 2, organs were harvested and IVIS® imaged. Mice circled (#17, 24, 27, 32, and 39) were not injected to serve as luciferase background controls. Only using high sensitivity Bin 10 resolution, low bioluminescence signals (indicated with arrows) were detected in mice 21, 22 and 38 of vector groups A, C and Mut-4 respectively.

FIGS. 11(A and B). Tumor targeting of Ar-339 E2 mutants. A) SCID female mice were i.p. injected on day 0 with 2×10⁶ ES-2 cells/mouse. On day 4, five mice per group were i.p. injected with one dose of vector A (JT-BB/SP6-RFluc), vector C (SP6-H/SP6-RFluc), Mut-1(SP6-H-K70/SP6-RFluc), Mut-2(SP6-H-K70-V251/SP6-RFluc) or Mut-4(SP6-H-13-K70-E181-V251/SP6-RFluc), and the next day (day 5) IVIS® imaged for vector luciferase signals. The peritoneum was removed for imaging of the peritoneal cavity and the organs were harvested and imaged. Vectors A and C efficiently targeted tumors, Mut-4 showed low bioluminescence signals, Mut-1 and Mut-2 did not show luminescence. For the three mutants, IVIS® images at Bin 10 resolution were taken of full mice and organ arrays. Only at this high sensitivity did some mice of Mut-1 and Mut-2 groups show very low residual signals in metastatic implants (lower panels), arrows point to regions with signals. B) Some mice per group received a second i.p. injection of the vectors on day 6, and peritoneal cavities and organs were IVIS® imaged on day 7. One mouse per group (#80, 85, 89, 94 and 99) was not injected to serve as luciferase background controls. For the three E2 mutants, high sensitivity Bin 10 images were also taken. Vectors showed equivalent infection pattern as for first injection.

FIGS. 12 (A and B) Colocalization in peritoneal cavity of vector Mut-4. A) Vector Mut-4 infection colocalized with the metastasized ES-2/Fluc tumors in the peritoneal cavity as determined by the IVIS® Imaging System. SCID mice were i.p. inoculated with 1.5×10⁶ ES-2/Fluc cells. Five days later, while the disease was still microscopic, inoculated mice received a single i.p. treatment of vector Mut-4 and were imaged the next day. The first IVIS® imaging was done by i.p. injection of Rluc substrate, coelenterazine, followed by a 5-minute acquiring interval (left panel). Thirty minutes after the coelenterazine injection, when the short-lived Rluc signals faded away, Fluc substrate, D-luciferin, was i.p. injected to determine the ES-2/Fluc tumor locations (right panel). B) Correlation analysis of vector Mut-4 shows a high correspondence between tumor cells and vector infection in the peritoneal cavity.

FIG. 13. (A-C) Suppression of disease progression by Ar-339 and Mut-4 chimeric vectors. A) ES-2/Fluc cells (1.5×10⁶) were i.p. inoculated into SCID mice on day 0. The next day (day 1), mice were imaged using the IVIS® Imaging System with D-luciferin as substrate and were split into four groups of five mice each: control which received no vector treatment, vector D, vector C and vector Mut-4. The groups received daily i.p. treatments of corresponding Sindbis vectors (10⁶ TU) and were IVIS® imaged on days 1, 5, 13 and 19 after the start of treatment. All vector treatments suppressed the tumor growth on the mesentery and diaphragm and reduced the signals on the omentum compared with control mice. Image scale Min 8×10³ Max 10⁵ counts/pixel. B) Quantitative analysis of the whole-body total photon counts of control and Sindbis-treated mice. Error bars represent the SEM. C) Survival curve of mice.

FIG. 14 is a Restriction endonuclease map of the DM-Helper plasmid.

DETAILED DESCRIPTION OF THE INVENTION

The term Aabout@ or Aapproximately@ means within an acceptable error range for the particular value as determined by one of ordinary skill in the art, which will depend in part on how the value is measured or determined, i.e., the limitations of the measurement system, i.e., the degree of precision required for a particular purpose, such as a pharmaceutical formulation. For example, Aabout@ can mean within 1 or more than 1 standard deviations, per the practice in the art. Alternatively, Aabout@ can mean a range of up to 20%, preferably up to 10%, more preferably up to 5%, and more preferably still up to 1% of a given value. Alternatively, particularly with respect to biological systems or processes, the term can mean within an order of magnitude, preferably within 5-fold, and more preferably within 2-fold, of a value. Where particular values are described in the application and claims, unless otherwise stated the term “about” meaning within an acceptable error range for the particular value should be assumed.

In accordance with the present invention there may be employed conventional molecular biology, microbiology, and recombinant DNA techniques within the skill of the art. Such techniques are explained fully in the literature. See, e.g., Sambrook, Fritsch & Maniatis, Molecular Cloning: A Laboratory Manual, Second Edition. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press, 1989 (herein “Sambrook et al., 1989”); DNA Cloning: A Practical Approach, Volumes I and II (D. N. Glover ed. 1985); Oligonucleotide Synthesis (M. J. Gait ed. 1984); Nucleic Acid Hybridization [B. D. Hames & S. J. Higgins eds. (1985)]; Transcription And Translation [B. D. Hames & S. J. Higgins, eds. (1984)]; Animal Cell Culture [R. I. Freshney, ed. (1986)]; Immobilized Cells And Enzymes [IRL Press, (1986)]; B. Perbal, A Practical Guide To Molecular Cloning (1984); Ausubel, F. M. et al. (eds.). Current Protocols in Molecular Biology. John Wiley & Sons, Inc., 1994.

Amino acid residues in proteins are abbreviated as follows: Phenylalanine is Phe or F; Leucine is Leu or L; Isoleucine is Ile or I; Methionine is Met or M; Valine is Val or V; Serine is Ser or S; Proline is Pro or P; Threonine is Thr or T; Alanine is Ala or A; Tyrosine is Tyr or Y; Histidine is His or H; Glutamine is Gln or Q; Asparagine is Asn or N; Lysine is Lys or K; Aspartic Acid is Asp or D; Glutamic Acid is Glu or E; Cysteine is Cys or C; Trytophan is Trp or W; Arginine is Arg or R; and Glycine is Gly or G.

As used herein, the term Atumor@ refers to a malignant tissue comprising transformed cells that grow uncontrollably. Tumors include leukemias, lymphomas, myelomas, plasmacytomas, and the like; and solid tumors. Examples of solid tumors that can be treated according to the invention include sarcomas and carcinomas such as, but not limited to: fibrosarcoma, myxosarcoma, liposarcoma, chondrosarcoma, osteogenic sarcoma, chordoma, angiosarcoma, endotheliosarcoma, lymphangiosarcoma, lymphangioendotheliosarcoma, synovioma, mesothelioma, Ewing's tumor, leiomyosarcoma, rhabdomyosarcoma, colon carcinoma, pancreatic cancer, breast cancer, ovarian cancer, prostate cancer, squamous cell carcinoma, basal cell carcinoma, epidermoid carcinoma, adenocarcinoma, sweat gland carcinoma, sebaceous gland carcinoma, papillary carcinoma, papillary adenocarcinomas, cystadenocarcinoma, medullary carcinoma, bronchogenic carcinoma, renal cell carcinoma, hepatoma, bile duct carcinoma, choriocarcinoma, seminoma, embryonal carcinoma, Wilms' tumor, cervical cancer, testicular tumor, lung carcinoma, small cell lung carcinoma, bladder carcinoma, epithelial carcinoma, glioma, astrocytoma, medulloblastoma, craniopharyngioma, ependymoma, pinealoma, hemangioblastoma, acoustic neuroma, oligodendroglioma, meningioma, melanoma, neuroblastoma, neuroglioma, and retinoblastoma.

The phrase Apharmaceutically acceptable@, as used in connection with compositions of the invention, refers to molecular entities and other ingredients of such compositions that are physiologically tolerable and do not typically produce untoward reactions when administered to a human. Preferably, as used herein, the term Apharmaceutically acceptable@ means approved by a regulatory agency of the Federal or a state government or listed in the U.S. Pharmacopeia or other generally recognized pharmacopeia for use in mammals, and more particularly in humans.

The term Atherapeutically effective@ applied to dose or amount refers to that quantity of a compound or pharmaceutical composition that is sufficient to result in a desired activity upon administration to a mammal in need thereof. As used herein with respect to viral vectors of the invention, the term Atherapeutically effective amount/dose@ refers to the amount/dose of a vector or pharmaceutical composition containing the vector that is sufficient to produce an effective anti-tumor response upon administration to a mammal.

Described below are preferred replication defective Sindbis viral vectors for use in the present invention termed vector C, Mut-1, Mut-2 and Mut-4 and the plasmids used to produce them. Mut-1, Mut-2 and Mut-4 contain mutations in the E2 envelope protein and are alternatively referred to herein as “E2 Mutants”.

The E2 mutants of the present invention were produced using unique helper and replicase plasmids. The present invention provides four novel helper plasmids (SP6-H (SEQ ID NO:37) SP6-H-K70 (SEQ ID NO:38), SP6-H-K70-V251 (SEQ ID NO:40) and SP6-H-I3-K70-E181-V251 (SEQ ID NO:39)) and one replicon plasmid (SP6-R (SEQ ID NO:36)). Helper plasmid SP6-H, which does not contain any amino acid changes but has a different nucleotide sequence, is set forth in SEQ ID NO:37. The 4 helper plasmids are used to produce Vector C, Mut-1, -2 and -4 vectors, respectively, when produced using the novel replicon plasmid.

In order to produce viral vectors in this system, two plasmids are used, the replicon and the helper. The replicon contains the viral replicase, the viral packaging signal, nt 945 to nt 1075, (Weiss B et al. 1994; Frolova et al 1997); the viral subgenomic promoter, multicloning site I (MCS1) to allow for the insertion and expression of the gene of interest, and the 3′ end of the virus (nt 11394 to 11703) to allow viral (−) strand RNA synthesis. A second multicloning site (MCS2) allows for the linearization of the plasmid for in vitro transcription.

The helper plasmid contains the first 425 nt of the virus, followed by the 3′ end of the virus from nt 7334 to nt 11703 which includes the subgenomic promoter, the capsid and the viral envelope proteins (E3, E2, 6K and E1) and the 3′ end (nt 11394 to 11703).

Both plasmids share the following viral sequences: the first 425 nt and the 309 nt of the 3′ end and the sub genomic promoter.

Both plasmids have several non-viral elements in common, the replication origin (rep pMB1) and the Ampicillin resistance gene from the pUC cloning plasmid; the promoter for in vitro transcription (T7 or SP6) and the MCS2. In the construction process a plasmid containing the pUC sequences, SP6 or T7 promoter, the multicloning sites, and the 3′ viral end, which are common to both vectors, was first generated. The specific viral sequences were then cloned into this plasmid (FIG. 3).

In order to produce the viral vectors of the present invention, one pair of plasmids are linearized using restriction enzymes such as PacI, NotI, or XhoI, transcribed in vitro, the RNAs collected and electroporated into cells. For in vitro transcription, a promoter is inserted before the Sindbis viral sequences. Preferably, the promoter is a bacteriophage promoter, for us with its respective RNA polymerase such as SP6 or T7 (Ambion Austin, Tex.).

Cells for use in the present invention include BHK-21 cells (available from the American Type Culture Collection, ATCC, Manassas, Va. as CRL 6281), ES-2 cells, (ATTC, CRL 1978), ES-2/Fluc cells that were derived from the ES-2 line by transfection of a plasmid, pIRES2-Luc/EGFP and the MOSEC cell line (clone ID8). The transcribed RNAs (i.e., one helper and one replicon plasmid) are electroporated into the cells at a concentration ranging between about 0.75 mg/mL and about 1.25 mg/mL. The ratio of viral RNA to cell concentration ranges between about 30-50 μg RNA per 6×10⁶ cells. Electroporation is performed using equipment commercially available from e.g., Bio Rad (Hercules, Calif.)

The transfected cells are fed medium containing 5% fetal bovine serum (FBS) and incubated at 37° C. for about 12 hours. The medium is then discarded, replaced with 9 ml of Opti-MEM I medium (GIBCO-BRL Invitrogen, San Diego Calif.) and incubated at about 37° C. for about 24 hours. Then, supernatants are collected and centrifugated at 2,400 rpm (≈1,500 g) for 15 min to eliminate cell debris. Clear supernatants, containing the viral vector, were collected, aliquoted and stored at −80° C.

The viral vectors can be used as anti-tumor agents as described in International Application No. PCT/US02/09432 published as WO 02/076468. Although the Mut-1 vectors do not bind to the high affinity laminin receptors (HALR), they are useful as controls and to monitor non-viral effects of the vectors. Since Mut-1 vectors do not enter cells, the contribution of host factors in the anti-cancer response can be studied.

The plasmids of the present invention can be used to transfer of cells and create packaging cell lines for the continuous production of defective Sindbis viral vectors as described in copending Ser. No. 10/983,432 and in Paper Example 1 below.

The amount of viral vectors produced may be determined as described below in the Examples. Briefly, clear supernatants are serial diluted in Opti-MEM I medium and 300 μL of each vector dilution are added to a 35 mm well in 12-well plates, containing 2×10⁵ cells. After incubation for 1 hour at room temperature, the cells are washed with PBS (Phosphate buffered saline) and incubated with 2 mL of αMEM at 37° C. for about 24 hours. Media is removed, cells are washed with PBS and cell lysates are prepared and assayed for the different reporter activity: β-galactosidase, firefly luciferase or Renilla luciferase. Vector titers were estimated as the highest dilution having detectable reporter activity. Detection of reporter activities is described in Example 2.

Viral vectors can be produced by linearizing helper and replicon plasmids after the polyA sequence, followed by separately performed in vitro transcription reactions. Usually, 1.6 μg of plasmid yields 15-25 μg of mRNA/reaction. Then 30-50 μg of both RNAs are co-electroporated into 6×10⁶ BHK-21 cells, which are then incubated in 10 ml of αMEM containing 5% FBS at 37° C. for about 12 h. Then, the medium is replaced with 9 ml of Opti-MEM I medium (GIBCO-BRL Invitrogen, San Diego Calif.) supplemented with 0.7 μM CaCl₂, cells are incubated at 37° C. for about 24 h, supernatants collected and centrifuged at ≈1,500 g for 15 min to eliminate cell debris. The procedure can be scaled up using the following electroporation ratios: 5-8 μg helper and replicon mRNA's per 10⁶ BHK-21 and 9 mL Ca-Opti-MEM media per reaction.

The viral vectors of the present inventions have the following properties which are summarized in Strauss and Strauss 1994. Briefly, as Alphaviruses replicate exclusively in the cytoplasm, there is no possibility of adventitious splicing. Because they are replication incompetent and packaging defective, the vectors are incapable of spread by reinfection. The vector replicates to high copy number inside the cell, and large quantities of mRNA are produced, leading to production of large amounts of the protein of interest

Viral vectors obtained as described herein can be formulated in a pharmaceutical composition for administration to a patient. As used herein, a Apharmaceutical composition@ includes the active agent, i.e., the viral vector, and a pharmaceutically acceptable carrier, excipient, or diluent. The phrase Apharmaceutically acceptable@ refers to molecular entities and compositions that are physiologically tolerable and do not typically produce an allergic or similar untoward reaction, such as gastric upset, dizziness and the like, when administered to a human. Preferably, as used herein, the term Apharmaceutically acceptable@ means approved by a regulatory agency of the Federal or a state government or listed in the U.S. Pharmacopeia or other generally recognized pharmacopeia for use in animals, and more particularly in humans. The term Acarrier@ refers to a diluent, adjuvant, excipient, or vehicle with which the compound is administered. Such pharmaceutical carriers can be sterile liquids, such as water and oils, including those of petroleum, animal, vegetable or synthetic origin, such as peanut oil, soybean oil, mineral oil, sesame oil and the like. Water or aqueous saline solutions and aqueous dextrose and glycerol solutions are preferably employed as carriers, particularly for injectable solutions. Suitable pharmaceutical carriers are described in “Remington's Pharmaceutical Sciences” by E. W. Martin.

For human therapy, the viral vectors of the present invention may be prepared in accordance with good manufacturing process (GMP) standards, as set by the Food & Drug Administration (FDA). Quality assurance (QA) and quality control (QC) standards will include testing for replication competent virus (if the virus vector is replication defective), activity (colony forming units [CFU] per number of viral particles, tested by induction of apoptosis or cytopathic effect (CPE), or by expression of a marker gene such as β-galactosidase), toxicity, and other standard measures.

In order to treat the tumors, the pharmaceutical composition is administered by any route that will permit homing of the vector to the tumor cells. Preferably, administration is parenteral, including, but not limited to, intravenous, intra-arteriole, intramuscular, intradermal, subcutaneous, intraperitoneal, intraventricular, and intracranial administration. As disclosed herein, viral vectors can be also administered to the tumor-bearing animal via intranasal or oral route (see Hardy, In: The Arbiviruses: Epidemiology and Ecology, Ch. 4, pp. 87-126). Importantly, however, in contrast to other viral vectors in gene therapy, administration of the Sindbis vectors of the invention need not be locally to the tumor. Indeed, one of the advantages of this invention is the high specificity and affinity of the vector for cancer cells, even micrometastases that cannot be resected or located by standard techniques (e.g., CAT scanning, MRI scanning, etc.).

In the therapeutic treatments of the invention, a therapeutically effective amount of the vector is administered to a patient. As used herein, the term Atherapeutically effective amount@ means an amount sufficient to reduce by at least about 15 percent, preferably by at least 50 percent, more preferably by at least 90 percent, and most preferably prevent, a clinically significant deficit in the activity, function and response of the host. Alternatively, a therapeutically effective amount is sufficient to cause an improvement in a clinically significant condition in the host. Specifically, a therapeutically effective amount will cause one or more of the following: apoptosis of tumor cells; necrosis of tumor cells; elimination or prevention of tumor metastases; reduction in the rate of tumor growth; reduction in tumor size or tumor shrinkage; scab formation over cutaneous tumor; elimination of the tumor; remission of the cancer; an increase in the time for reappearance of the cancer; and increased time of survival of the patient. The frequency and dosage of the vector therapy can be titrated by the ordinary physician using standard dose-to-response techniques, but generally will be in the range of from about 10⁶ to about 10¹² viral vector particles per dose administered daily, weekly, biweekly, or monthly at least twice and preferably at least three times.

In order to explore if wild type Sindbis would improve the vector systems, four vectors were generated combining the helper and replicase segments of both strains (JT-BB/SP6-R; SP6-H/JT-Rep; SP6-H/SP6-R; JT-BB/JT-Rep) and tumor size reduction and survival in tumor-induced SCID mice was studied. Unexpectedly, it was found that those vectors carrying Ar-339 helper (SP6-H) were less efficient in targeting tumors than the JT vectors and that those carrying Ar-339 replicase (JT-BB/SP6-R and SP6-H/SP6-R) were more efficient in tumor reduction. To study the surprising phenotype of the helper, both viral sequences were analyzed and compared. One amino acid difference was found in the capsid protein (Pro₆₇ (JT-BB) to Gln (Ar-339)); three amino acid changes in envelope protein E1 (Ala₇₂ (JT-BB) to Val (Ar-339), Gly₇₅ (JT-BB) to Asp (Ar-339) and Ser₂₃₇ (JT-BB) to Ala (Ar-339)) and four changes in envelope protein E2 (Ile₃(JT-BB) to Thr(AR-339); Lys₇₀(JT-BB) to Glu(Ar-339); Glu₁₈₁(JT-BB) to Lys(Ar-339) and Val₂₅₁(JT-BB) to Ala(Ar-339)). To determine which amino acids were critical for the vector properties, different chimeras from both vectors were generated containing mixed sequences from both strains. The analysis in animal models showed that the Ar-339 E2 envelope protein sequence was primarily responsible for tumor metastases targeting although the optimum amino acid pattern was not defined. To address this question, vectors were generated containing sequences with mixed combinations of the four amino acids in E2, for JT and Ar-339, and tested by IVIS® imaging in ES-2 induced and tumor-free mouse models. Surprisingly, the change of only one amino acid from Glu to Lys at position 70 of Ar-339 E2, suppressed the ability to target metastatic tumor implants in mice and also showed no fat tissue background. This result was unexpected, considering that this mutant (Mut-1) repeatedly showed titers equivalent to the Ar-339 vector when measured in ES-2 cultured cells. Double mutant Mut-2, with K70 and V251 did not revert the targeting. Only when the whole sequence of JT E2 was substituted in the Ar-339 helper was the ability to target metastatic tumor implants recovered, a though with less intensity. Thus, residue 70 in the outer leaf of the E2 protein is essential for tumor specific targeting of vectors.

Described herein are amino acids of Sindbis viral vectors involved in specific infection of metastatic tumor implants in the mouse peritoneal cavity. Sequence analysis of cloned Ar-339 and JT viral vectors has been an important tool for the discovery of these amino acids.

The sequence analysis comparison with the published Strauss sequence, revealed 2 or 3 amino acid changes in viral replicase for JT and Ar-339 respectively. The Ar-339 nsP1 Ile. 441 is a reverse mutation to an alphavirus conserved residue; this amino acid may also be related to viral adaptation to BHK-21 tissue culture. Ar-339 replicase is more efficient in suppression of disease progression than JT, the change from polar Cys in JT to aliphatic residue, Ile 441, may play a role in enhancing replicase activity. Interestingly, both JT and Ar-339 vectors have the same 2 amino acid changes in nsP2 protein versus the Strauss sequence. JT and Ar-339 nsP2 residues are more conserved among the Sindbis-like Alphavirus group.

Comparing the Strauss sequence with the region coding for the structural part of the JT and Ar-339 vectors, the changed amino acids were found mainly in the viral spike, although in different residues, suggesting a different evolutionary lineage of both strains. E1 D75 and A237 of Ar-339 are highly conserved in Sindbis-like alphaviruses; all viruses in this group carry D75. There is no virus in the group that has serine at 237, which only occurs in the JT vector. These data suggest that E1 G75 and S237 of JT vector may more likely be point mutations that arose in this laboratory strain. Further studies with E1 mutants should reveal the implication of these E1 residues in the specific tumor targeting of the viral vector. Most of the sequence variabilities have been found at the E2 envelope protein in the leaf-like domain at the viral spike; these residues are also poorly conserved in the Sindbis-like alphavirus group. The role of these E2 spike mutations in mouse tumor models in vivo was examined.

The fact that the Mut-1 vector shows the same titer as the Ar-339 vector in ES-2 cells, but does not efficiently infect ES-2 metastatic tumor implants in mice, represents a powerful tool for the study and the improvement of Sindbis vectors for gene therapy. One cause of the loss of tumor targeting in vivo could be a reduced stability of the vector in mice. Alignment of protein sequences among 17 different viruses of the Sindbis-like alphavirus group showed that ten out of the 17 members have a gap in Sindbis E2 residues 68-71, including Semliki Forest virus (SFV) which has a comparable structure to Sindbis virus. The viral spike is composed of three E1-E2 heterodimers that lean against each other. There is a gap between the base of neighboring E1-E2 heterodimers which would allow E2 to move out of the center of the spike during fusion (Zhang et al. 2002). In addition, previous studies with stable deletion mutants in the E2 receptor binding domain, also show equivalent in vitro titers but drastic reduction of infectivity in live Aedes aegypti mosquitoes (Myles et al. 2003). Without wishing to be bound by theory, it is believed that these data suggest that there is structural flexibility in this area of the spike, therefore, alterations in residue 70 shouldn't be critical for Mut-1 vector stability in vivo. The loss of vector infectivity would be more likely to occur via a decrease in cell binding affinity, especially in vivo, where the environmental conditions for vector infection are more restrictive.

In Sindbis and Semliki Forest virus (SFV), the residues involved in host cell fusion and binding to cellular receptors are located in the viral spike. It has been described that only one change from the small non-polar Valine, at position 75, to the acidic Aspartic acid in E1 SFV spike subunit, modifies the cell-cell fusion properties of the virus (Levy-Mintz and Kielian, 1991). Amino acids in these E1 and E2 spike domains are, thus, important in spike configuration and virus infectivity.

The vector Ar-339, having hydrophobic (V72), and acidic (D75) residues in E1 and a glutamic acid (E70) in E2 protein, is able to efficiently target tumor cells in vivo. In Mut-1, in which E2 residue 70 is changed to lysine, there is a change in polarity and charge of the amino acid that would change the conformation of the spike and so the cell binding properties of the vector. This hypothesis is supported by the difference in cell tropism observed in vitro between Mut-1 and Ar-339. Only the recovery of the full sequence of JT-BB E2 in Mut-4 results in higher titers in BHK-21 cells. If these differences were observed in vitro, where the conditions for cell binding are optimized, it is possible that in vivo factors involved in cell-vector interactions might be able to prevent vector adherence to the viral receptor. Ar-339 and Mut-1 vectors have the same titer in ES-2 cells in vitro, but in mouse metastatic implants, where ES-2 cells are in a different environments and could have receptor variations, small affinity differences between both vectors are revealed.

The present invention is described further below in examples which are intended to describe the invention without limiting the scope thereof.

In the examples below, the following materials and methods were used.

Example 1 Sindbis cDNA Cloning

Common Techniques

Virus Provocation

Sindbis virus strain Ar-339 (Original) was obtained from ATCC (Manassas, Va., Item #VR-68) and propagated on a secondary chicken embryo fibroblast cell line, CEF. Cells were cultured in EMEM media (BioWhittaker, cat#12-684) supplemented with 10% Fetal bovine sera, NaHCO₃ 1.5 g/l; 1-Glutamine, 292 mg/l and Penicillin/Streptomycin, 100 U/ml. Two T-75 flasks with 80% confluent CEF cell monolayers, were infected with 2.5×10⁷ pfu and 5×10⁷ pfu, respectively, of Sindbis virus Ar-339, diluted in 1 ml of Opti-Mem I media (GIBCO-BRL Invitrogen, San Diego Calif.; cat#31985-070) supplemented with 0.7 μM CaCl₂. Virus-infected cells were incubated at 37° C. for 1 h, 10 ml of EMEM media/flask added, and cells were incubated overnight at 37° C. Supernatants containing the Sindbis “innocula” were harvested and stored at −80° C. until used in further infections. CEF cells were collected for total RNA extraction.

RNA Extraction

5 T-75 flasks with 90% confluent CEF monolayers were incubated 1 h at 37° C. with 1:100; 1:20; 1:10; and 1:5 dilutions (in Ca-OPTI-MEM media) of previously obtained “Sindbis innocula”. Ten ml of EMEM media/flask was then added and incubated overnight at 37° C. After collecting the supernatant, 2 ml of Trizol (Invitrogen, San Diego, Calif.; cat #15596-018) per flask was added to the infected cells, the extract collected and stored at −80° C. Total RNA from infected cells was prepared following the manufacturer's (Trizol) protocol. Briefly, 1 ml of Trizol cell extract was vortexed for 15 seconds, 200 μL of chloroform added, vortexed, spun at 15,000 g for 10 min at room temperature (rt), the aqueous upper phase transferred to a clean tube, 750 μL of isopropanol added, incubated at rt for 15 min, spun again, the supernatant removed and the pellet washed with ethanol (70% in DEPC water). The pellet was air dried at rt for 5-10 min, and resuspended in 50 μL of DEPC water.

Sindbis c-DNA Cloning. Sindbis virus RNA was cloned in 6 overlapping fragments (CDNA-1, CDNA-2, CDNA-3A, CDNA-3-B, CDNA-4 and CDNA-5) into sequencing plasmid pCR4Blunt-TOPO (Invitrogen, San Diego Calif.; cat#45-0245). The position of the fragments is shown in FIG. 1 and the sequences of the primers used are shown in Table I (Appendix A). The primers were designed to take advantage of the unique restriction sites of the virus. In primer SV-C3R RE XbaI was introduced to allow CDNA-3 cloning. For each fragment the RT and the PCR reactions were performed with the same pair of primers. The cloning procedure was the same for all of the fragments except for the conditions of the PCR cycles.

Reverse transcriptase (RT) reactions were performed with: 5 μg from total RNA from infected cells, reaction buffer (1×); forward and reverse specific primers (2.5 μM/each); dNTPs (1 mM): DTT (5 mM) and 15 u of ThermoScript™ RNase H-Reverse Transcriptase (Invitrogen, cat#12236-014), in a final volume of 20 μL. RT was diluted 1:1 in distilled water prior to use in PCR.

The Pfx-PCR reactions were performed with 1 μL of RT reaction, reaction buffer (1×), MgSO₄ (1 mM), dNTPs (0.3 mM/each), forward and reverse primers (0.3 μM/each) and 1 U Platinum Pfx DNA Polymerase (Invitrogen, cat#11708-013) in final volume of 25 RT and PCR reactions were performed in an Eppendorf “Mastercycler Gradient” Thermal Cycler. Taq-PCR reactions contained reaction buffer (1×); dNTP's (200 μM), forward and reverse primers (0.5 μM/each) and 1 u Taq-DNA-polymerase (Fisher Scientific, Pittsburgh, Pa., cat#FB 600025) in a final volume of 20 μl. In both cases PCR products were analyzed by electrophoresis in 1% agarose gels and DNA bands cut out and purified with a QIAEXII gel extraction kit (Qiagen, Valencia Calif.; cat#20021)

All enzymes were purchased from New England Biolabs (NEB, Beverly, Mass.). After digestions with restriction enzymes (RE), extracted DNAs were CIP dephosphorylated (1 h at 37° C.) and/or phosphorylated with T4-Polynucleotide-Kinase (1 h, 37° C.), enzymes were inactivated (70° C., 10 min or 65° C. 20 min) and reactions were run in 1% agarose gels. Bands were cut out, extracted, and quantified by gel electrophoresis by comparison with bands of known DNA concentration. Ligations were carried out with T4-DNA-Ligase (NEB) (16° C. 14 h) or with the Quick ligation kit (NEB) (5 min at room temperature (rt)). Transformations were performed using RapidTrans™ TAM1 extra competent E. coli (Active Motif, Carlsbad, Calif.; cat#11099). After transforming E. coli with the ligations, the first screening of positive bacteria was done by Taq-PCR of E. coli colonies. Positive plasmids were checked by restriction enzymes. Sequencing was done at the Skirball Institute of Biomolecular Medicine, NYU.

CDNA-1 plasmid. RT reaction was performed with primers CDNA-1F/CDNA1-R at 55° C. 1 h. Two Pfx-PCR were performed: 94° C. 5 min; 35 cycles 194° C. 30 s; 66° C., 30 s (band D) or [67° C., 30 s; 72° C., 2.5 min and 72° C., 2.5 min] (band E). PCR products were run in 1% agarose gels and both 2.2 Kb bands, D and E, were isolated and cloned separately into the pCR4Blunt-TOPO plasmid. Positive colonies were screened using the same PCR conditions with 65° C. as the annealing temperature and Taq-DNA-Polymerase. Two plasmids were selected (one from each PCR band) and the PCR band D in plasmid CDNA-1_Topo#64 was completely sequenced. The sequence was compared to the Ar-339 sequence published by Strauss et al., Virology 133, 1984. The differences found were compared with the sequences of PCR band E. Identical differences were found in bands D and E to confirm that they came from the virus and not from PCR mutations.

CDNA-2 plasmid. The Reverse transcriptase reaction was performed with primers CDNA-2F/CDNA-2R for 1 h at 55° C. Three PCR in gradient 94° C., 5 min; 35 cycles of [94° C., 30 s; 64.7° C., 30 s (band 1); or 66° C., 30 s (band 2); or 67° C., 30 s (band 3); 72° C., 2.5 min] and 72° C., 2.5 min. After cloning the 2 kb bands separately in pCR4Blunt-TOPO, PCR screening of colonies was done at an annealing temperature of 64.5° C. Band 2 in plasmid CDNA-2#213 was fully sequenced, and any mutations found were checked by comparison with the sequences of PCR bands 1 and 3.

CDNA-3A plasmid. Three RT reactions were performed in gradient with primers CDNA-3F/SV-6932R at 53.2° C., 55.5° C. and 60.8° C. respectively for 1 h 30 min. Three Pfx PCR were performed with 1 μL of each RT, respectively: 94° C., 5 min; 35 cycles of [94° C., 30 s; 53° C., 30 s; 72° C., 3 min] and 72° C. 3 min. The 2.68 kbp bands T, U and V were cloned separately and band V in plasmid C3A_Topo#735 was fully sequenced. No changes were found compared with the published Ar-339 sequences (Strauss et a1.1984).

CDNA-3B plasmid. RT reaction was performed with primers SV-6882F/SV-C3R at 60.8° C. for 1 h 30 min. Three Pfx-PCR in gradient were performed with 94° C., 5 min; 35 cycles of [94° C., 30 s; 55.3° C., 30 s (band J); or 56° C., 30 s (band K); or 58.3° C. 30 s (band L); 72° C., 1 min] and 72° C., 2 min. The three 774 by bands were cloned separately and band L, in plasmid C3B-Topo#334, was fully sequenced. The mutations found were confirmed by sequencing bands J and K.

CDNA-3 (CDNA-3A+3B) plasmid. HpaI RE is not unique in the Sindbis sequence, so in order to clone CDNA3A and CDNA3B fragments into the new reporter vector, it was necessary to generate first a Topo plasmid containing both sequences, and then clone fragment CDNA-3(CDNA-3A+3B) in the AvrII/XbaI site. Plasmid C3B-Topo#334 was HpaI and SacI digested and the 774 by viral band was isolated from the agarose gel and ligated (T4-DNA-Ligase 16° C. 14 h) to HpaI/SacI digested C3A_Topo#735 plasmid. Transformants were screened by PCR with Taq-Polymerase, and primers CDNA-3F, SV-C3R at: 94° C., 10 min; 35 cycles of [94° C. 30 s; 55° C., 30 s; 72° C., 3 min] and 72° C. 2 min. The positive plasmid was named C3A+B_Topo#810, and the junctions were sequenced.

CDNA-4 plasmid. The RT reaction was performed with primers CDNA-4F/CDNA4-R at 52° C. for 1 h 30 min. Three PCRs were performed in gradient with 94° C., 5 min; 35 cycles of [94° C., 45 s; 49.7° C., 30 s (band M); or 55° C., 30 s (band N); or 57.2° C., 30 s (band O); 72° C. 2.5 min] and 72° C. 2.5 min. The 2 kbp bands were isolated and cloned separately, band N in plasmid CDNA-4_Topo#422 was fully sequenced. The mutations found were confirmed by sequencing bands M and O.

CDNA-5 plasmid. RT reactions performed with primers CDNA-5F/CDNA5-R at 49.7° C. for 1 h 30 min. Three PCR were made in gradient with 94° C., 5 min; 35 cycles of [94° C., 30 s; 46.8° C., 30 s (band Q); or 48.6° C., 30 s (band R); or 50.5° C., 30 s (band S); 72° C. 2 min] and 72° C., 2 min. The 2.35 kbp bands were cloned separately and band S in plasmid CDNA-5_Topo#525 was fully sequenced. The mutations found were confirmed by sequencing bands Q and R.Vectors construction.

Polylinker cloning in pUC plasmid. In summary, the SP6 and T7 Polylinker bands were constructed out of primers in two fragments, a 5′ end containing the SP6 promoter (primer pairs Poly1-SP6(+)/Poly-2(−)) or T7 promoter (primers Poly1-T7(+)/Poly-2(−)) and a common 3′end (primers Poly-3(+)/Poly-4(−)) the sequences of the primers are given in Table II (Appendix A). 5′ and 3′ bands were digested and ligated to generate the polylinker and then were ligated to the AflIII/AatII pUC band containing the E. coli replication origin and Ampicillin resistance gene to generate T7-pUC or SP6-pUC plasmids.

T7-pUC plasmid. Two separate reactions were performed with primers pairs Poly1-T7(+)/Poly-2(−) or Poly-3(+)/Poly-4(−). For both reactions, conditions were: primers (5 μg each), buffer (1×), dNTPs (1.5 mM), BSA (50 μg/ml). Mixes were boiled for 5 min and cooled down to room temperature for primer annealing, 3 units/reaction of T4-DNA-Polymerase was then added to a final volume of 20 μl, and incubated at 37° C., for 30 min for chain extension. 0.5 μL of each reaction was used as a template in two PCR reactions: one with template Poly1-T7(+)/Poly-2(−) and primers PCR-Poly1-F/PCR-Poly2-R and other with template Poly-3(+)/Poly-4(−) and primers PCR-Poly-3F/PCR-Poly-4R. For both PCRs, the conditions were: reaction buffer (1×), dNTP's (200 μM), forward and reverse primers (0.5 μM/each) and 1 unit of Taq-DNA-polymerase in a final volume of 20 μl. For each primary reaction, 3 secondary tubes were prepared and PCR reactions were performed in gradient for annealing temperature: 94° C., 5 min; 35 cycles of [94° C., 30 s; 40° C., 30 s (tube 1); or 45.4° C., 30 s (tube 2); or 50.7° C., 30 s (tube 3); 72° C., 30 s] and 72° C. 1 min. Bands were stronger for the 40° C. annealing temperature. The 145 bp bands were then isolated and digested with enzymes: AflIII and XbaI for PolyT7/Poly2-R and, AatII and XbaI for Poly3(+)/Poly 4(−). After inactivating the enzymes at 65° C. 20 min, the ligation of equimolar amounts of both bands was carried out at 25° C. for 1 h with T₄-DNA-ligase. The 1811 nt AatII/AflII band of the pUC plasmid was added, and incubated at 16° C. overnight. E. coli Tam1 competent cells were transformed with the ligations and positively selected colonies screened by double digestion with AatII and AflIII. The positive plasmid was named T7-pUC#32 and was checked by sequencing.

SP6-pUC plasmid. The reaction mix: Poly1-SP6(+) and Poly-2(−) primers (5 μg each), buffer (1×), dNTPs (1.5 mM), BSA (50 μg/ml), was boiled for 5 min and cooled down to rt for primer annealing. Then 3 units/reaction of T4-DNA-Polymerase was added to a final volume of 20 μl, and incubated at 37° C., 30 min. 0.5 μl/Rxn was used as a template in a gradient PCR reaction with PCR-Poly1-F/PCR-Poly2-R primers (0.5 μM/each) reaction buffer (1×), dNTP's (200 μM), and 1 unit of Taq-DNA-polymerase in a final volume of 20 μl. PCR conditions: 94° C., 5 min; 35 cycles of [94° C., 30 s; 40° C., 30 s (tube 4); or 45.4° C., 30 s (tube 5); or 50.7° C., 30 s (tube 6); 72° C., 30 s] and 72° C. 1 min. Bands were isolated in agarose gels, pooled together and purified. The DNA obtained was digested with enzymes AflIII and XbaI for 2 h at 37° C. After inactivating the enzymes at 65° C., 20 min, the digested DNA band was ligated to the AatII and XbaI digested Poly-3(+)/Poly-4(−) band at 25° C. for 1 h with T4-DNA-ligase. Five μL of the ligation was used as the template for a second gradient PCR reaction carried out with primers PCR-Poly1-F and PCR-Poly4-R, (same concentrations as previous PCR) and cycles: 94° C. 2 min, 18 cycles of [94° C., 30 s; 40° C., 30 s (tube 4); or 43.1° C., 30 s (tube 5); or 45.4° C., 30 s (tube 6); 72° C. 30 s] and 72° C. 1 min. Bands were isolated in an agarose gel, pooled together, purified and AftIII/AatII digested at 37° C. for 16 h. After enzyme inactivation (65° C., 20 min), the AflIII/AatII SP6 band was ligated to the 1811 nt AatII/AflII band of the pUC plasmid with Quick T4-DNA-ligase (New England Biolabs) at rt for 5 min and transformed into E. coli Tam1 cells. Screening of colonies was performed as for T7 polylinker. The plasmid was named SP6-pUC#51.

T7-pUC-PolyA#114 plasmid. The Sindbis 3′ end from nt 11392 to 11694 was obtained by PCR on plasmid CDNA-5_Topo #525 with primers: PolyA-F (5′ CCCCAATGATCCGACCA 3″) (SEQ ID NO:1) and PolyA-R (5′ AAAACAAATTTTGTTGATTAATAAAAG 3′) (SEQ ID NO:2). PCR conditions: reaction buffer (1×), MgSO₄ (1 mM), dNTPs (0.3 mM each), primers (0.3 μM each) and 1 unit of Platinum Pfx DNA Polymerase in a final volume of 25 μl. Three PCR reactions were performed in gradient for annealing temperature: 94° C., 5 min; 35 cycles of [94° C., 45 s; 53.2° C., 30 s (tube 1); or 55.5° C., 30 s (tube 2); or 60.8° C., 30 s (tube 3); 72° C. 45 s] and 72° C. 1 min. The 53.2° C. band was stronger and its DNA was isolated from the gel, phosphorylated with 10 units of T4-Polynucleotide-Kinase (Biolabs) at 37° C., 30 min. After inactivation at 70° C. 10 min, the DNA was ligated to plasmid T7-pUC#32 (previously digested with HpaI and dephosphorylated) using T₄-DNA-ligase at 16° C. for 14 h. Screening of recombinants was performed by PCR of colonies using the same primers, Taq-Polymerase and cycles: 94° C., 5 min; 35 cycles of [94° C., 45 s; 53° C., 30 s; 72° C., 45 s] and 72° C. 1 min. The orientation of the insert was analyzed with restriction enzymes AflII/AseI. The positive plasmid was confirmed by sequencing, and named T7-pUC-PolyA#114. From this vector the final vector T7-ARep#68 and SP6-pUC-PolyA#914 was generated to construct the final vector SP6-Arep#68.

Replicon vector constructions. T7-ARep plasmid: Viral cDNA fragments CDNA-1, CDNA-2, and CDNA-3 were cloned in T7-pUC-PolyA#114 to generate a new reporter vector T7-ARcp#68. Plasmid CDNA-1_Topo#64 was digested with MfeI and BglII RE and the 2247 bp viral band isolated and ligated, with the quick ligase kit, to T7-pUC-PolyA#114, MfeI, BglII digested and CIP dephosphorylated. TAM1 transformant bacteria were screened by PCR with Taq-Polymerase and primers CDNA-1F and CDNA-1R at: 94° C. 10 min, 35 cycles of [94° C., 30 s; 65° C., 30 s; 72° C. 2.5 min] and 72° C. 2 min. The positive plasmid was named T7-pUC-PolyA-C1#11 and was AvrII/BglII digested and CIP dephosphorylated and ligated, with Quick ligase, to the CDNA-2 1950 by viral band, obtained after AvrII/BglII digestion of plasmid CDNA-2_Topo#213. Screening of positive colonies was made by PCR with Taq polymerase and primers CDNA-2F and CDNA-2R at: 94° C., 10 min; 35 cycles of [94° C., 30 s; 64° C., 30 s; 72° C. 2.5 m] and 72° C. for 3 min. The plasmid was named T7-pUC-PolyA-C1-C2#35. C3A+B_Topo#810 was digested with XbaI/AvrII and BsshII, the 3350 nt viral C3 band separated in an agarose gel, isolated and ligated (Quick Ligase Kit) to XbaI/AvrII digested T7-pUC-PolyA-C1-C2#35 plasmid. Transformants were analyzed by XbaI/AvrII digestion. The new reporter vector was named T7-ARep#68, and was fully sequenced.

SP6-ARep plasmid. The reporter vector under the SP6 promoter was cloned in three steps. First, the SP6 promoter was cloned into T7-pUC-PolyA#114, then CDNA-1 was inserted and in the last step, the CDNA-2+CDNA-3 band from T7-ARcp#68 was cloned.

SP6-pUC#51 was SphI and AflIII digested and the 154 nt band isolated and ligated (T4-DNA-ligase) to the T7-pUC-PolyA#114 SphI/AflIII/CIP band. Plasmids were screened by MboII digestion and checked by sequencing. The positive plasmid was named SP6-pUC-PolyA#902, and was digested with MfeI and BglII, CIP dephosphorylated and ligated (quick ligase) to the MfeI/BglII CDNA-1 band. Colonies were analyzed by PCR with CDNA-1F/CDNA-1R primers at: 94° C. 10 min, 35 cycles of [94° C., 30 s; 65° C., 30 s; 72° C., 2.5 min] and 72° C. 2 min. The positive plasmid was also analyzed by MfeI/BglII digestion and named SP6-pUC-PolyA-C1#306. Plasmid T7-ARep#68 was BglII/XbaI digested, and a 5.6 kb CDNA-2+CDNA-3 band was isolated from an agarose gel and ligated to BglII/XbaI/CIP digested SP6-pUC-PolyA-C1#306 plasmid. Screening was done by BglII and BglII/XbaI digestions. The new vector was named SP6-Arep#701.

Helper vector constructions. 77-AH#17 plasmid. In a first step Sindbis virus nts 1 to 425 were cloned into T7-pUC-PolyA#114. In a second step, both CDNA-4 and CDNA-5 viral fragments were cloned to generate a new helper vector. Sindbis nt 1 to 425 were amplified by PCR using as a template 54 ng of CDNA-1_Topo#64, primers (0.5 μM/each) SIN1-19F (5′ ATTGACGGCGTAGTACACA 3′) (SEQ ID NO:3) and H-BamR (5′GTATCAAGTAGGATCCGGAG 3′) (SEQ ID NO:4) which adds a BamHI RE to allow CDNA-4 fragment cloning, reaction buffer (1×), dNTP's (200 μM), and 1 unit of Taq-DNA-polymerase in final volume of 20 μl. Two PCR reactions were performed in gradient for the following annealing temperature: 94° C. 5 min, 28 cycles of [94° C., 30 s; 45.3° C., 30 s (tube 1); or 46.4° C., 30 s (tube 2); 72° C., 30 s] and 72° C. 1 min. Bands were analyzed in agarose 1.3% gels, extracted, pooled together and digested with MfeI/BamHI. Plasmid T7-pUC-PolyA#114 was MfeI/BamHI digested and CIP dephosphorylated, the band isolated from an agarose gel and ligated to the 350 by MfeI/BamHI PCR band. Colonies were screened by Taq-PCR with primers SIN1-19F/H-BamR at: 94° C., 10 min; 45 cycles of [94° C., 30 s; 45° C., 30 s; 72° C. 30 s] and 72° C. 1 min. A positive plasmid was checked by sequencing and named T7-pUC-PolyA-5′#604. This plasmid was digested with BamHI and NsiI and CIP dephosphorylated to ligate with viral inserts.

The ligation of CDNA-4 and CDNA-5 was through RE BclI. This enzyme is dam methylation dependent, so to demethylate the DNA, plasmids harboring CDNA-4_Topo#422 and CDNA-5_Topo#525 were transformed into dam⁻/dcm⁻ E. coli strain GM2163 (New England Biolabs). CDNA-4_Topo#422(dam−) was BamHI and BclI digested and plasmid CDNA-5_Topo#525 was digested with BclI and NsiI. In both cases, the enzymes were inactivated at 70° C. for 15 min. The ligation was performed with T4-DNA-Ligase (16° C. for 14 h) with equimolar amounts of the three bands: T7-pUC-PolyA-5′#604 (BamHI/NsiI/CIP), CDNA-4_Topo#422(dam⁻) (BamHI/BclI) and CDNA-5_Topo#525(dam⁻)(BclI/NsiI). Colonies were screened by BamHI and NsiI digestion. The positive plasmid was named T7-AH#17 and was fully sequenced.

SP6-AH plasmid. Plasmid SP6-pUC-PolyA#902 was digested with MfeI and NsiI, CIP treated and the 2.4 kb band was ligated (T4-DNA-ligase) to the 4.5 kb T7-AH#17 MfeI/NsiI band. Colonies were screened by Taq PCR with primers CDNA-5F and CDNA-5R at: 94° C., 10 min; 25 cycles of [94° C., 30 s; 53° C., 30 s; 72° C. 3 min]. Positive plasmids were checked by NsiI, and NsiI/MfeI digestions and sequenced. The resulting plasmid was named SP6-AH#318

T7-R AND SP6-R plasmids. The four plasmids (SP6-AH#318, SP6-ARep#701, T7-AH#17 and T7-ARep#68) were fully sequenced, and in all four, a deletion of one T at the 3′ end of the virus before the polyA, nt 11686 was found. In order to have the same sequence as the virus Ar-339, the deletion was fixed.

The Ar-339 sequence was placed first in plasmid T7-pUC-polyA#114. The new 3′ end was obtained by PCR with primers: PolyA-F (5′ CCCCAATGATCCGACCA 3″) (SEQ ID NO:5) and END-R (5′ AAAACAAAATTTTGTTGATTAATAAAAG 3′) (SEQ ID NO:6) and cloned into T7-pUC#32, as described previously for T7-pUC-polyA#114 cloning. The new plasmid T7-pUC-3end#9 was sequenced and used to generate new helpers and reporters.

To generate the reporter vectors, the T7-pUC-3end#9 plasmid was digested with XbaI and XhoI and the 423 by band was cloned into the SP6-Arep#701 XbaI/XhoI 9504 by band and into the T7-ARep#68 XbaI/XhoI 9504 by band to generate new reporters SP6-R#406 and T7-R#202, respectively.

T7-H AND SP6-H plasmids. Plasmid T7-pUC-3end#9 was digested with NsiI and XhoI and the 311 by band was cloned into the SP6-AH#38 NsiI/XhoI 6399 by band and into the T7-AH#17 NsiI/XhoI 6399 by band, to generate, respectively, the new helpers SP6-H#432 and T7-H#226. The new reporters and helper plasmids were fully sequenced.

SP6-HE2 mutants. Mutants were made on SP6-H plasmid following the kit QuickChange IIx site-directed mutagenesis (Stratagem, La Jolla, Calif.). Briefly, 10 ng of SP6-H#432 were incubated with complementary primers: E2-I3-F/E2-I3-R or E2-K70-F/E2-K70-R or E2-E181-F/E2-E181-R or E2-V251-F/E2-V251-R, reaction buffer, dNTPs and 2.5 units of pfuUltra HF DNA polymerase. PCR reactions were 95° C. 1 min, 18 cycles of: 95° C. 50 s, 60° C. 50 s, 68° C. 7 min, and final elongation of 68° C. 7 min.

After the PCR reactions 10 U of restriction enzyme DpnI was added and reaction incubated 37° C. 1 hour to digest methylated parental DNA. XL-10-Gold competent E. coli cells were transformed and the colonies analyzed by restriction enzyme digestion (RE). Mutations were verified by sequencing. Multiple mutants were made following the same protocol using previous mutants as template for PCR. The sequence of primers used and nt changes are shown in Table III (Appendix A). Plasmids made: SP6-H-13#5; SP6-H-K70#59; SP6-H-I3-K70#66; SP6-H-I3-K70-E181#3; SP6-H-K70-V251# SP6-H-I3-K70-E181-V251. In order to make sure that there were not additional mutations due to the PCR technique, the 1270 by BssHII/BanII fragment of each clone was subcloned in the sequenced plasmid SP6-H.

Reporter gene cloning in replicon vectors. The LacZ gene was cloned in the PmlI/XbaI site of vectors SP6-ARep#701, SP6-R#406, T7-ARep#68 and T7-R#202 to generate SP6-ARepLacZ, SP6-RLacZ, T7-ARepLacZ and T7-RLacZ, respectively.

The firefly luciferase gene was excised at the NheI/XbaI sites from pGL3 plasmid (Promega Co, Madison Wis.) and cloned in the XbaI site of vectors: SP6-ARep#701, SP6-R#406, T7-ARep#68 and T7-R#202 to generate SP6-ARepFluc, SP6-RFluc, T7-ARepFluc and T7-RFluc, respectively.

The Renilla luciferase gene from plasmid phRL-CMV (Promega Co, Madison Wis.) was cloned in the XhaI site of the replicons, to generate plasmids: SP6-RhRluc and T7-RhRluc.

Example 2

Cells. BHK-21, and ES-2 cells were obtained from the American Type Culture Collection (ATCC). BHK-21 cells were maintained in αMEM (JRH Bioscience) with 5% FBS. ES-2 cells were derived from a patient with clear cell carcinoma, a type of ovarian cancer that has a poor prognosis and is resistant to several chemotherapeutic agents including cisplatin. ES-2 cells were cultured in McCoy's 5 A medium (Mediatech) with 5% FBS. All basal media were supplemented with 100 μg/ml of penicillin-streptomycin and 0.5 μg/ml of amphotericin B (both from Mediatech). ES-2/Fluc cells were derived from the ES-2 line by transfection of a plasmid, pIRES2-Luc/EGFP, that expresses a bicistronic mRNA transcript containing both firefly luciferase and EGFP genes. To construct the pIRES2-Luc/EGFP plasmid, a DNA fragment containing the luciferase gene was obtained from pGL-3 basic plasmid (Promega) and then subcloned into the multicloning sites of the pIRES2-EGFP plasmid (BD Biosciences Clontech).

The mouse ovarian MOSEC cell line (clone ID8) was a generous gift from Dr. Katherine F. Roby (University of Kansas Medical Center, Kansas City) and was maintained in Dulbecco's modified Eagle's medium (DMEM) supplemented with 4% FBS and 1× insulin-transferrin-selenium (Mediatech, Inc).

In vitro transcription and vector preparation. The plasmids carrying the Sindbis replicon or JT-BB helper RNAs were linearized with PacI, NotI or XhoI, before in vitro transcription using the mMESSAGE mMACHINE RNA transcription kit (T7 and SP6 version; Ambion). Both helper and replicon RNA transcripts (20 μl each) were then electroporated into BHK-21 cells and incubated in 10 ml of αMEM containing 5% FBS at 37° C. for 12 h. The medium was replaced with 9 ml of Opti-MEM I medium (GIBCO-BRL, Invitrogen San Diego Calif.) supplemented with 0.7 μM CaCl₂. After 24 h, the culture medium was collected and stored at −80° C.

Vector titering. The titers of Sindbis vectors were assayed in BHK-21, ES-2, ES-2/Fluc or MOSEC cells. Serial dilutions (300 μL each) of vector were added to 2×10⁵ BHK-21 cells in 12-well plates. After incubation for 1 hour at room temperature, the cells were washed with PBS and incubated with 2 mL of αMEM at 37° C. for 24 hours.

LacZ expression was determined by two methods: staining and counting blue cells/well or reading absorbance. For the first, cells were fixed in PBS containing 0.5% glutaraldehyde at room temperature for 20 minutes, washed three times with PBS, and then stained with PBS containing 1 mg/mL X-gal (5-bromo-4-chloro-3-indolyl-D-galactopyranoside; (Fisher Scientific, Pittsburgh, Pa.), 5 mM potassium ferricyanide, 5 mM potassium ferrocyanide, and 1 mM MgSO4 at 37° C. for 3 hours. After staining with the X-Gal solution, cells that expressed LacZ were blue. Blue-stained cells were counted and vector titers were estimated by determining the number of transducing units (TU) per mL of aliquot. With the second method, cells were lysed with 200 μL of M-PER lysis buffer (Pierce Biotechnology, Rockford, Ill.). 50 μL of the cell lysates were added into 50 μL of All-in-One-Galactosidase Assay Reagent (Pierce Biotechnology) and incubated at room temperature for 5 minutes before reading at 405 nm. Vector titers were estimated as the last dilution having detectable absorbance.

Firefly luciferase activity in cell lysates was determined by aspirating the culture medium from the wells and adding 200 μL per well of culture medium and 200 μL per well of Steady-Glo™ reagent (Promega Corp., Madison, Wis.). Plates were incubated with gentle rocking for 5 minutes until the cells detached from the plates. The cell lysates were then transferred to 12×47 mm cuvettes (BD Pharmingen, San Diego, Calif.), and the luciferase activity of each lysate was determined by taking a 30-second reading with a LUMI-ONE portable luminometer (Bioscan, Inc., Washington, D.C.).

Renilla luciferase activity was determined by following the protocol in “Renilla Luciferase assay system” (Promega Corp., Madison, Wis.). Briefly, cells were washed with PBS and 250 □l/well of lysis buffer was added. 20 μL of substrate were added to 100 μL of extract and the luciferase activity of each lysate was determined by taking a 30-second reading with a LUMI-ONE portable luminometer (Bioscan, Inc., Washington, D.C.).

Animal models. All animal experiments were done in accordance with NIH and institutional guidelines. To determine the therapeutic effects of Sindbis virus vectors, SCID mice (female, 6-8 week old; Taconic, Germantown, N.Y.) were i.p. injected with 1.5×10⁶ ES-2/Fluc cells/mouse on day 0 and imaged with the IVIS® system the next day (day 1) to confirm the presence of tumor cells. Then on day 4, 5 mice/vector received i.p. treatment with vectors carrying the Renilla luciferase: A (JT-BB/SP6-RhRluc), B (SP6-H/JT-RephRluc), C (SP6-H/SP6-RhRluc), D (JT-BB/JT-RephRluc). All vectors had the same titer in ES-2/Fluc cells, and mice were i.p. injected daily with ˜10⁶ TU in 0.5 ml Opti-MEM l/mouse. Control mice (n=5) received no Sindbis vector treatment. Disease progression was later determined by IVIS® imaging on days 1, 5, 9 and 13.

Survival curves were compared with log rank test. All the P values presented in this study are two-tailed.

For colocalization experiments, two SCID mice/vector were i.p. inoculated with 2×10⁶ ES-2/Fluc cells on day 0 and received one i.p. treatment of vector C (˜10⁶ TU in 0.5 mL of OptiMEM I) on day 5. The next day (day 6), mice were i.p. injected with 0.3 mL of 0.2 mg/mL coelenterazine (Biotium, Inc., Hayward, Calif.) followed by IVIS® imaging for Renilla luciferase activity. 30 minutes later, the same mice were i.p. injected with 0.3 mL of 15 mg/mL D-luciferin (Biotium, Inc) and a second IVIS® imaging for Firefly luciferase activity was performed.

Cell tropism experiments, were done in two groups of SCID mice with 5 mice/vector: one without tumor induction and the second one in mice with induced tumors.

The tumor free animals were i.p. injected with Fluc vectors on day 0, imaged by IVIS® on day 1, received a second injection of vectors on day 2 and on day 3 were IVIS® imaged again.

For the second group, SCID female mice were injected i.p. on day 0 with 2×10⁶ ES-2 cells/mouse and on day 4 mice were injected with vectors carrying the luciferase reporter genes. After the first whole-body IVIS® imaging on day 1, the peritoneum was removed for another IVIS® imaging of the peritoneal cavity. The remaining mice of the group, except one for background control, had a second i.p. injection of vectors on day 6 and imaged again on day 7.

In vivo bioluminescence detection with the IVIS® Imaging System. A cryogenically cooled IVIS® Imaging System Series 100 (Xenogen) was used with Living Image acquisition and analysis software (Version 2.11, Xenogen Corp. Alameda, Calif.) to detect the bioluminescence signals in mice. For firefly luciferase detection, each mouse was injected i.p. with 0.3 ml of 15 mg/ml beetle luciferin (potassium salt; Promega Corp., Madison, Wis.) in PBS. After 5 min, mice were anesthetized with 0.3 ml of avertin (1.25% of 2,2,2-tribromoethanol in 5% tert-amyl alcohol) or isofluran-mixed oxygen. The imaging system first took a photographic image in the chamber under dim illumination; this was followed by luminescent image acquisition. The overlay of the pseudocolor images represents the spatial distribution of photon counts produced by active luciferase. An integration time of 1 min was used for luminescent image acquisition for all mouse tumor models. Living Image software (Wave Metrics Inc., Lake Oswego, Oreg.) was used to integrate the total bioluminescence signals (in terms of proton counts) obtained from mice. The in vitro detection limit of the IVIS® Imaging System is 1,000 ES-2/Fluc cells.

Ar-339 Sequence Analysis

Ar-339 virus was amplified in chicken embryo fibroblasts and cloned into sequencing plasmids as six separate overlapping fragments (FIG. 1). CDNA-3B and CDNA-4 overlap the 312 by fragment (nt 7334-7646) that contains the viral subgenomic promoter. In order to avoid mutations due to RT or PCR reactions, for each plasmid three different RT reactions were performed and each one served as template for one PCR reaction. The three PCR amplified bands of each fragment were cloned separately, sequenced and compared to verify the virus sequence. The Ar-339 sequence obtained was compared to the Sindbis published sequence (Strauss EG, Rice CM and Strauss JH 1984) and to the sequence of Sindbis vectors that we used previously in our laboratory, JT-BB and JT-Rep. (Tseng et al 2004a,b). The results are shown in Table IV (Appendix A). The differences in sequence between the Strauss sequence and JT vectors are described in Table V (Appendix A). Functional changes between JT and AR-339 plasmids are summarized in Table VI (Appendix A).

In the viral replicase, comparing the Strauss map with Ar-339, three point mutations in nsp1 were found: nt 353 a silent mutation; nt 1380 and 1381. Both change amino acid 441 from Cys to Ile in Ar339. In the Sindbis-like virus supergroup, the methyltransferase nsp1 has four conserved motifs I (a.a 31 to 51), II (a.a 87 to 86), III (168 to 220) and IV (243 to 260) (Rozanov et al. 1992). Cys 441 to Ile is not in the carboxyterminal domain required for enzymatic activity (K468 to L512) (Wang et al. 1996). Nsp2 has three mutations compared to the Strauss sequence, one silent at nt 3698 (A to G) and two (nt 2992 and 3579) that change amino acids 438 (Pro to Leu) and 634 (Lys to Glu) respectively. Both amino acids are outside the active helicase and protease domains of nsp2 (Rikkonen et al. 1994). Sindbis virus with Pro at 438, as described in the Strauss sequence, has lethal effects on virus replication (Rice et al. 1987). In nsp4 there was a silent mutation at nt 7337 (T to C).

Regarding the structural proteins, the Ar-339 capsid protein had two mutations compared with the Strauss sequence, one silent at nt 8345 (C to A) and one at nt 7846 that changed Pro 67 to Gln. In the consensus Strauss sequence and JT vectors a Proline occurs at position 67. This residue is conserved in different isolates of virus in Australia, and for MK6962, a New Guinea isolate, a Tyr (T) is present at this site (Sammels et al. 1999). This change is in the 11 to 74 amino acid region that doesn't bind to Sindbis RNA (Geigenmuller-Gnirke et al. 1993) and is not in E2 or capsid proteins interaction domains residues 36-39, 108 to 111, 172, 180 to 183, 201, 231-234, 240 or 254 (Katherine E. Owen and Richard J. Kuhn, 1996; H. Lee and D. T. Brown 1994).

There were also two silent mutations in E1 at nt 10392 (T to C) and 10469 (T to A) and two differences in the Ar-339 with the Strauss map were found at positions Ala 72 to Val in Ar-339 and 237 (Ser to Ala), which are both located in domain II. Residues of this domain are involved in E1-E1 interaction in the virus spike (Zhang W et al. 2002).

Most of the coding changes were found in the envelope protein E2, in which the antigenic sites and the binding receptor domain of the virus have been described. Comparing the Strauss sequence with Ar-339, five amino acid changes were found located in the external leaf-like domain of the E2 protein, which extends from the amino terminus to residue 218 (Zhang W et al. 2002). Changes are in amino acids: 3 (Ile to Thr); 23 (Val to Ala); 70 (Lys to Glu) and also two of mutations, 172 (Arg to Gly) and 181 (Glu to Lys), occur in the putative binding receptor domain (amino acids 170 to 220). No changes were found in the endodomain that interacts with the capsid protein (from 391 to 483) or with the E2-E1 interaction region.

An analysis of the amino acid changes between the JT plasmids and the Ar-339 sequence revealed only one mutation in the replicase, Cys 441 to Ile of nsp1. In the structural proteins there were a total of eight differences, only one in the capsid protein, Pro 67 to Gln; and seven in the E1 and E2 envelope proteins. Three mutations in E1: Ala 72 to Val; Gly 75 to Asp and Ser 237 to Ala. Most of the differences were found in the E2 protein, three in the leaf-Like domain; Ile 3 to Thr; Lys70 to Glu and Glu 181 to Lys and one in the ectodomain, Val 251 to Ala. V251 is important for virus maturation in CEF (Li M L et al. 1999).

Vector Constructions

To construct the Ar-339 vectors, the Sindbis genome was split into two plasmids: the replicon and the helper (FIG. 2). This vector system is designed to electroporate in vitro transcribed viral RNA into the susceptible cell line to produce replicative defective Sindbis virus, called viral vector, that contains, as a genome, the replicase RNA and lacks the structural genes. For in vitro transcription, a bacteriophage promoter is required before the viral sequence.

In order to compare the yield of viral vectors in this system, two pairs of vectors were produced, one pair with replicon and helper under the control of the SP6 promoter (SP6-H and SP6-R), and the other pair under the control of the T7 promoter (T7-H and T7-R).

The replicon contains the viral replicase, with the packaging signal, nt 945 to nt 1075, (Weiss B et al. 1994; Frolova et al 1997); the viral subgenomic promoter, multicloning site 1 (MCS1) to allow for the insertion and expression of the gene of interest, and the 3′ end of the virus (nt 11394 to 11703) to allow viral (−) strand RNA synthesis. A second multicloning site (MCS2) allows the linearization of the plasmid for in vitro transcription.

The helper plasmid contains the first 425 nt of the virus, followed by the 3′ end of the virus from nt 7334 to nt 11703 which includes the subgenomic promoter, the capsid and the viral envelope proteins (E3, E2, 6K and E1) and the 3′ end (nt 11394 to 11703).

Both plasmids share the following viral sequences: the first 425 nt, the 309 nt of the 3′ end and the sub genomic promoter.

Both plasmids have several non-viral elements in common, the replication origin (rep pMB1) and the Ampicillin resistance gene from the pUC cloning plasmid; the promoter for in vitro transcription (T7 or SP6) and the MCS2. In the construction process a plasmid containing the pUC sequences, SP6 or T7 promoter, the multicloning sites, and the 3′ viral end, which are common to both vectors, was first generated. The specific viral sequences were then cloned into this plasmid (FIG. 3).

SP6 and T7 Promoters

For in vitro transcription systems, the RNA yield using SP6 or T7 RNA polymerase for long RNA transcripts could differ. To study if the promoter would make a difference in the titer of the viral vectors production, BHK-21 cells were electroporated with two sets of in vitro transcribed RNAs: SP6-AH and SP6-ARepLacZ, to generate the SP6-LacZ viral vector, and with T7-AH and T7-ARepLacZ for the T7-LacZ vector. The comparison of both vector's titers in BHK-21 cell, in repeated experiments, gave equivalent titers ≈10⁶ transducing units (TU)/mL. In terms of infective particles production, both promoters work with the same efficiency in this system.

Sequencing of the four plasmids (SP6-AH, SP6-ARep, T7-AH. and T7-ARep) revealed a deletion of one T at the 3′ end of the virus before the poly A (SV nt 11686). To study the effect of this deletion on viral vectors, four new plasmids without the deletion were constructed (SP6-H, SP6-R, T7-H and T7-R) and titers of viral vectors from both sets of plasmids were compared. No significant difference was observed indicating that the deletion of T11686 is not critical for vector replication. As these four sets of vectors showed the same in vitro titer, in order to standardize results the experiments with mice were performed using SP6-H and SP6-R plasmids to synthesize viral vectors.

Example 3 Biological Properties

Cell Tropism

Most of the amino acid differences found between JT and Ar-339 vectors were in the envelope proteins. One of them was related to virus adaptation to BHK-21, E2 Lys 70 to Glu (McNight K et al, 1996) and two of them were located in the receptor-binding domain of the E2 protein. To analyze if the amino acid changes had any effect on the viral vector's infectivity, JT, Ar-339 and chimeric viral vectors were produced and titered in three cell lines: BHK-21 (baby hamster kidney), ES-2 and Mosec human and mouse ovarian cancer cell lines respectively. The results are shown in Table VII (Appendix A). Vectors that carry JT-BB helper (JT-BB/SP6-ARepLacZ and JT-BB/JT-RepLacZ) had titers two logarithms higher in BHK-21 than in the other two cell lines; when the helper was SP6-H, the difference observed was only one log. The infectivity of the vectors in vitro was similar in both ovarian cancer cell lines, ES-2 and Mosec. This difference was observed in repeated experiments.

Disease Progression

In order to compare the ability in targeting and suppression of disease progression by Sindbis vectors Ar-339 and JT, JT and Ar-339 chimeric vectors were produced and tested in the ES-2/Fluc mouse metastatic ovarian cancer model described previously (Tseng et al. 2004b). Five female SCID mice per vector group were injected i.p. with 1.5×10⁶ ES-2/Fluc cells (day 0) and IVIS® imaged the next day to verify the presence of ES-2/Fluc cells. Cells were left to grow for four days before daily treatment with vectors was started. There were five mouse groups, one of which did not receive vector treatment, whereas the remaining 4 had vectors: A(JT-BB/SP6-RhRluc), B (SP6-H/JT-RephRluc), C (SP6-H/SP6-RhRluc) and D (JT-BB/JT-RephRluc). As these strains showed different cell tropism in BHK-21 cells, vectors were titered in the same cell line used to induce the tumor ES-2/Fluc, and titers for all vectors were standardized at 10⁶ TU/mL. Total whole body photon counts were determined by IVIS® imaging on days 1, 5, 13, and 19 to determine disease progression of ES-2/Fluc metastases (FIG. 4). Survival curves were also compared (FIG. 5).

Vector A, carrying the Ar-339 replicase (SP6-RhRluc) and JT structural proteins was more efficient in reducing tumor progression and gave better survival of the animals. The vectors carrying the same structural proteins, vector B versus C and A versus D were compared. In both cases there was more tumor reduction with Ar-339 replicase (SP6-RhRluc). Regarding the structural part, when the vectors carrying the same replicase were analyzed, in one case SP6-H seemed to be more effective (Vectors D versus B), but in the case of A versus C, where the photon count difference is larger, JT-BB is more efficient in tumor targeting. The small differences between vectors B, C and D correlates with similar animal survival data, although both structural proteins and replicase function in the efficiency of the vectors in vivo, these data suggest that an improvement in the targeting of Ar-339 replicase (SP6-R) to tumor cells would lead to more efficient gene therapy vectors.

Co-Localization

To establish the degree and specificity of Ar-339 Sindbis infection of tumor cells, IVIS® imaging studies were performed that measured independent bioluminescent signals from tumor cells and vectors. The ES-2/Fluc cells expressed the firefly luciferase gene, that uses D-luciferin as substrate, and the vectors carried a different luciferase gene cloned from soft coral Renilla renifomis (Rluc) that uses coelenterazine to generate bioluminescence. The two luciferases are highly substrate specific and do not cross-react (Bhaumik S and Gambhir S S. 2002). Each anesthetized mouse was first treated with coelenterazine the image was collected (FIG. 6A, left panel), then treated with D-luciferin for a second IVIS® imaging, this time of ES-2/Fluc cells (FIG. 6A right panel). The bioluminescence signals generated in the same animal from Sindbis/Rluc and ES-2/Fluc, were quantitated using Living Image software. The images of Rluc and Fluc signals were grided (12 8, 96 boxed regions), and corresponding regions were analyzed for statistical correlation (FIG. 6B). A highly significant correlation was established (P<0.0001) indicating that a single i.p. delivery of Ar-339 Sindbis vector lead to the efficient infection of the metastasized tumor cells throughout the peritoneal cavity. In several mice an additional infection outside the peritoneal cavity was observed.

Ar-339 Targeting

To analyze tissues or organs targeted by the Ar-339 strain, new chimeric vectors were made with firefly luciferase (Fluc) as the reporter gene, since its stronger bioluminescent signal allows the study of vectors in animal organs. Each vector was tested in two groups of 5 SCID female mice: tumor-free and 5 day ES-2 metastasis induced mice. To assess which part of the C vector was responsible for the chest bioluminiscence, three Fluc chimeric vectors were made: A(JT-BB/SP6-RFluc), B(SP6-H/JT-RepFluc) and C (JT-BB/JT-RepFluc). As previously, vectors were titered in ES-2 cells. Tumor free mice received one dose of vector (10⁴ TU/mL) at day 0 and were IVIS® imaged next day (FIG. 7A). All three groups showed a low background signal in fat tissue. Two out of five vector B mice and one of five mice in vector C group showed some additional bioluminescent signal in the chest, as previously observed in the colocalization experiment. To investigate if vectors were infecting organs in these mice, intraperitoneal cavity and harvested organs were also IVIS® imaged. The chest signal observed corresponded to connective tissue in the ribs, while organs had no background signal. To study if repeated doses of these vectors could lead to accumulative infection in tumor-free mice, a second dose was i.p. injected on day 2 and the image repeated on day 3. The results (FIG. 7B) showed low background signal in fat tissue for vectors B and C and no signal at all for vector A, indicating that the background is transient and shouldn't affect the target effectiveness of Ar-339 vectors in repeated treatment.

Previous studies of JT/Fluc vector in 5 day ES-2 tumor induced mice, showed that the vector specifically targets metastasized ES-2 cells after one injection and also in a second dose two days later (Tseng et al. 2004). To study if the difference between sequences could affect the specificity of Ar-339 vector, these 3 chimeric vectors were tested in the same model. Mice injected with 2×10⁶ ES-2 cells on day 0, received one i.p. dose of vectors on day 5 and were IVIS® imaged on day 6. The peritoneal cavity and organs of two mice per group were imaged. As is shown in FIG. 8A for all vectors, bioluminescence correlated with ES-2 metastatic implants. At day 7 two mice per group received a second i.p. dose of vector and one mouse was not injected to serve as a luciferase background signal control (FIG. 8B). Vector A showed a similar signal compared with previous doses, but vectors carrying Ar-339 structural proteins, B and C, showed decreased bioluminescence signals in tumors compared with the first injection. The difference in reinfection suggests that amino acid changes in structural proteins could play an important role in targeting metastases by repetitive treatment with vector.

In order to determine which mutations were critical for the vector properties, a chimeric vector was generated, QE2, that contains E2 from JT-BB and the remaining structural proteins from Ar-339. When we compared with Ar-339 vector in the same IVIS® animal model as in the previous experiment, in tumor free animals, a low background in fat tissue with the first dose was observed and no signal in a second dose. In ES-2 5 day induced tumor mice, vector QE2 targeted tumor and was able to re-infect animals, though the bioluminescent signal was not as strong as for Ar-339 vector (data not shown). This indicates that Ar-339 sequence in the E2 envelope protein was primarily responsible for the targeting pattern, though the optimal amino acid pattern was still not clear. To address this question site directed mutagenesis was performed on the Ar-339 E2 envelope protein.

E2 Mutants

The E2 envelope has been described as the protein that is primarily responsible for cell tropism and infectivity of Sindbis virus. More specifically, Lys 70 is implicated in BHK-21 specificity (McNight K et al, 1996) and also residues 69 to 72 have been related to targeting vertebrate cells (Ohno K et al. 1997; Dubuisson J and Rice CM, 1993). As the Ar-339 strain has a Glu in this position, 3 point mutants were generated that contain Lys at position 70 (FIG. 9). In Mut-1 only Ar-339 Glu 70 was changed to Lys. Amino acid 251 of E2 is highly conserved between different Sindbis isolates (Sammels L M et al. 1999) and mutation to Valine at this position has been related with host range phenotype (Li M L et al. 1999). To explore the influence of this amino acid in vector targeting, Mut-2 was produced that has Lys70 and also Ar-339 Ala 251 mutated to Val. Residues at positions 3 and 181 are located in the external leaf-like domain of the E2 protein and 181 is in the receptor binding domain. To assess if the combination of these 4 amino acid changes in the E2 is responsible for the difference in infection of Ar-339 vectors, mutant Mut-4 was produced with Lys 70, and Val 251 plus changes in Ar-339 Thr 3 to Ile and Ar-339 Lys 181 to Glu (FIG. 9). The mutants were tested in tumor-free and ES-2 5 day induced tumor mice as previously described for vectors A, B and C. To assure that the difference in signals were due to the E2 mutations, all five vectors tested carried the same replicase SP6-RFluc: vector A (JT-BB/SP6-RFluc), C (SP6-H/SP6-RFluc), Mut-1 (SP6-H-K70/SP6-RFluc) Mut-2 (SP6-H-K70-V251/SP6-RFluc) and Mut-4 (SP6-H-13-K70-E181-V251/SP6-RFluc).

Vectors were titered in BHK-21 cells and in ovarian cell lines ES-2 and Mosec (Table VIII (Appendix A)). Mutants showed a greater difference between A and C when titered in BHK-21 than in ovarian cell lines.

In tumor-free mice vectors A and C gave background in some of the animals. Only one out of the five vector C mice showed a low bioluminescent signal in the ribs (FIG. 10A). In second doses of these vectors the background was even less noticeable (FIG. 10B). Mut-1 and Mut-2 did not produce background bioluminescent signal in either dose. Mut-4 showed barely detectable signal in fat tissue, much less intense than vectors A and C.

In ES-2 tumor induced mice (FIG. 11A) mice #79 in A and #88 in C vector groups did not show bioluminescence; these mice did not develop ES-2 metastatic implants in the peritoneal cavity (these mice are not shown in the Figure).

This result showed a dramatic decrease in the infectivity of mutants Mut-1 and Mut-2 and an important reduction in Mut-4 after the first and second dose (FIG. 11). The change of only one amino acid in Ar-339 E2 at position 70 makes vector C lose the specificity in targeting ES-2 tumor metastases in vivo. The double mutant with Valine at position 251, Mut-2, doesn't revert the vector tropism. Mut-4 combines vector A E2 with Ar-339 E1, E3 and capsid sequences. The fact that Mut-4 could not revert to full infectivity indicates that the interaction between E2 and E1 in the vector spike could also play an important role in vector targeting.

Example 4 Suppression of Disease Progression by Ar-339, MUT-4 and JT Vectors

In previous mouse experiments Vector C showed background infections in some of the animals treated. Although this effect was transient and vector C was efficient in suppression of disease progression, it would be preferable for gene therapy to use a viral vector that does not cause background tissue infections. In tumor targeting experiments of SP6-HE2 mutants, vector Mut-4 showed tumor targeting although with less intensity than vector C (FIG. 11) and did not show background infection (compare FIGS. 10A and 10B). With the aim of improving this vector system, the ability of Mut-4 in tumor reduction was studied and vector-tumor colocalization analyzed, using the same model described for vectors A, B, C and D as in previous experiments.

Materials and Methods

Animal models. All animal experiments were done in accordance with NIH and institutional guidelines. To determine the therapeutic effects of Sindbis virus vectors, SCID mice (female, 6-8 week old; Taconic, Germantown, N.Y.) were i.p. injected with 1.5×10⁶ ES-2/Fluc cells/mouse on day 0 and imaged with the IVIS® system the next day (day 1) to confirm the presence of tumor cells. Then on day 4, 10 mice/vector received i.p. treatment with vectors carrying the Renilla luciferase: Mut-4 (SP6-HI3K70E181V251/SP6-RHRluc), C (SP6-H/SP6-RhRluc) and D (JT-BB/JT-RephRluc). All vectors had the same titer in ES-2/Fluc cells, and mice were i.p. injected daily with ˜10⁶ TU in 0.5 ml Opti-MEM I/mouse. Control mice (n=5) received no Sindbis vector treatment. Disease progression was later determined by IVIS® imaging on days 1, 5, 9 and 13.

Survival curves were compared with log rank test. All the P values presented in this study are two-tailed.

For colocalization experiments, two SCID mice/vector were i.p. inoculated with 1.5×10⁶ ES-2/Fluc cells on day 0 and received one i.p. treatment of vector Mut-4 (˜10⁶ TU in 0.5 mL of OptiMEM I) on day 5. The next day (day 6), mice were i.p. injected with 0.3 mL of 0.2 mg/mL coelenterazine (Biotium, Inc., Hayward, Calif.) followed by IVIS® imaging for Renilla luciferase activity. 30 minutes later, the same mice were i.p. injected with 0.3 mL of 15 mg/mL D-luciferin (Biotium, Inc) and a second IVIS® imaging for Firefly luciferase activity was performed.

Results

Colocalization. To measure the degree and specificity of Mut-4 Sindbis infection of tumor cells, IVIS® imaging studies were performed that measured independent bioluminescent signals from tumor cells and vector Mut-4 (SP6-HI3K70E181V251/SP6-RhRluc). The ES-2/Fluc cells expressed the Firefly luciferase gene, that uses D-luciferin as a substrate, and the vector carried a different luciferase gene cloned from soft coral Renilla reniformis (hRluc) that uses coelenterazine to generate bioluminescence. The two luciferases are highly substrate specific and do not cross-react (Bhaumik, S. and Gambhir, S. S. 2002). Each anesthetized mouse was first treated with coelenterazine and the image was collected (FIG. 12A, left panel), then treated with D-luciferin for sequential IVIS® imaging of ES-2/Fluc cells (FIG. 12A, right panel). The bioluminescence signals generated in the same animal from vector and ES-2/Fluc were quantitated using Living Image software. The images of Rluc and Fluc signals were grided (12×8, 96 boxed regions), and the corresponding regions were analyzed for statistical correlation (FIG. 12B). A highly significant correlation was established (P<0.0001) indicating that a single i.p. delivery of Mut-4 Sindbis vector leads to very efficient infection of the metastasized tumor cells throughout the peritoneal cavity.

Disease progression. In order to compare the ability of Sindbis vectors Ar-339 and Mut-4 in targeting and suppression of disease, vectors were constructed and tested in the ES-2/Fluc mouse metastatic ovarian cancer model described previously. Ten female SCID mice per vector group were injected i.p. with 1.5×10⁶ ES-2/Fluc cells (day 0) and IVIS® imaged the next day to verify the presence of ES-2/Fluc cells in the mice. Cells were left to grow for four days before daily treatment with vectors was started. There were four groups of animals, one of which did not receive vector treatment, the remaining 4 were injected daily with 10⁶ TU/ml doses of vectors carrying Renilla luciferase reporter gene: C (SP6-H/SP6-RhRluc), Mut-4 (SP6-HI3K70E181V251/SP6-RhRluc). and D (JT-BB/JT-RephRluc).

Total whole body photon counts were determined by IVIS® imaging on days 1, 5, 13, and 19 to determine disease progression of ES-2/Fluc metastases. Survival curves were also compared (FIG. 13). Mice treated with vectors C and Mut-4 showed similar photo count reduction and survival proportions. These data suggest that vector Mut-4 (SP6-HI3K70E181V251/SP6-RHRluc) has similar in vivo efficiency in tumor reduction as vector C (SP6-H/SP6-RhRluc) and so can also be used in gene therapy. Both vectors, C and Mut-4, showed significantly improved tumor reduction and mouse survival compared to vector D.

Example 5 Background

The titer in BHK cells of defective Sindbis virus vectors C (SP6-H/SP6Rfluc), Mut-1 (SP6H-K70/SP6RFluc), Mut-2 (SP6H-K70-V251/SP6RFluc), and Mut-4 (SP6H-I3-K70-E181-V251/SP6RFluc) were lower than the titer of the vector carrying the JT-BB helper (Table A). The titer of the Mut-4 vector in BHK cells was ten times lower than the JT vector. Since all of the vectors carried the same replicon plasmid, the difference in titer should be due to the sequence of the Helper plasmid. In order to improve the production of the viral vectors, amino acid residues 72, 75 and 237 of the E1 protein in the Helper plasmid SP6H-I3-K70-E181-V251 were changed to Val, Asp and Ala respectively. These are the corresponding amino acids in the JT-BB Helper plasmid. The capsid residues were not modified. A summary of the differences in amino acid residues between these helpers is shown in Table D.

It has been disclosed that Helper RNAs with the tRNAAsp at the 5′ terminus are replicated to higher levels than RNAs with a 5′ terminus identical to that of Sindbis virus genomic RNA (U.S. Pat. No. 5,091,300). They also produced higher levels of PFU, indicating that they were packaged to higher levels than were Helper RNAs which had the Sindbis virus genomic 5′ terminus (20, 21). Because of this data, nt 11 to nt 73 of the AsptRNA sequence were cloned into the 5 end of the Helper vector.

DM-Helper Cloning.

The DM-Helper plasmid, was constructed from the SP6H-13-K70-E181-V251 plasmid (SEQ ID NO:39)) following 3 main steps: first, the AvrII restriction size was introduced in the 5′ end; second, E1 amino acid residues 72, 75 and 231 were mutagenized; third, the tRNA sequence in the AvrII site was added at the 5′ end. The sequence of primers used and nucleotide changes are shown in Table B.

1.—AvrII mutagenesis. The introduction of the AvrII site was performed using the QuickChange IIx site-directed mutagenesis kit (Stratagene, La Jolla, Calif.) using the manufacturer's instructions. Briefly, 10 ng of SP6H-13-K70-E181-V251 plasmid (SEQ ID NO:39) were incubated with its complementary primers: AvrII-F and AvrII-R, reaction buffer, dNTPs and 2.5 units of pfuUltra HF DNA polymerase. PCR reactions were 95° C. 1 min, 18 cycles of: 95° C. 50 s, 60° C. 50 s, 68° C. 7 min, and final elongation of 68° C. 7 min. After the PCR reactions 10 U of restriction enzyme DpnI was added and the reaction was incubated at 37° C. for 1 hour to digest methylated parental DNA. XL-10-Gold competent E. coli cells were transformed and the colonies analyzed by restriction enzyme digestion (RE). Mutations were verified by sequencing. The plasmid produced was named #810.

2.—E1 mutagenesis. The same QuickChange IIx site-directed mutagenesis kit (Stratagene, La Jolla, Calif.) was used to mutagenize consecutively E1 residues 72, 75 and 251. In the first reaction the complementary primers E1-7172F/E1-7172R were used yielding the plasmid #815. The second reaction was performed using 10 ng of plasmid #815 with primers E1-237F/E1-237R to obtain plasmid #821.

3.—Adding tRNA to 5′ end of vector 821, the T-125 plasmid. First the T-125 plasmid containing the tRNA sequence was generated, then tRNA was cloned into plasmid #821. Primer pairs TRNA-F(T-F)/TRNA-R(T-R)¹ (25 uM/each) were boiled for 1 min and cooled down to room temperature for primer annealing. This reaction (150 ng) was used as a template in the PCR reaction with primers T-F and T-R. The PCR conditions were: reaction buffer (1×), dNTP's (200 μM), forward and reverse primers (0.5 μM/each) and 1 unit of Taq-DNA-polymerase in a final volume of 20 μl. The PCR reaction was performed in three secondary tubes with a gradient for annealing temperature: 95° C., 5 min; 35 cycles of [95° C., 30 s; 52.1° C., 30 s (tube 1); or 55.2° C., 30 s (tube 2); or 58.5° C., 30 s (tube 3); 72° C., 30 s] and 72° C. 30 sec. Bands were stronger for the 52.1° C. annealing temperature. The 124 by bands were then isolated and ligated to the pCR®2.1-TOPO vector (Invitrogen, San Diego Calif.; cat#K45-0040) E. coli Tam1 competent cells were transformed with the ligation and positively selected colonies screened by double digestion with AvrII and MfeI. The positive plasmid was named T-125 and was checked by sequencing. ¹TRNA-F and TRNA-R are commercially available complementary oligonucleotides (Gene Link, Hawthorne, N.Y.). TRNA-E contains from the 5′ end the Avr Restriction Endonuclease (RE) site for cloning, the SP6 promoter, the tRNA sequence, the 5′ end of the Sindbis virus (SV) sequence (from nt29 to nt46) and the Mfe I RE site at the 5′ end.

Plasmid T-125 was digested with AvrII and MfeI, the 109 by band was isolated in an agarose gel, and ligated to the AvrII/MfeI band of the 821 plasmid. Screening of positive colonies was performed by digesting the plasmids with restriction enzymes AvrII/MfeI and the positive plasmid DM-helper#9 was fully sequenced.

Vector Production and Titering

Cells. BHK-21 were obtained from the American Type Culture Collection (ATCC Manassas, Va.). BHK-21 cells were maintained in αMEM (JRH Bioscience) with 5% FBS. Basal media was supplemented with 100 μg/ml of penicillin-streptomycin and 0.5 μg/ml of amphotericin B (both from Mediatech).

In vitro transcription and vector preparation. The plasmids carrying the Sindbis replicon SP6-R (SEQ ID No. 36) or DM-Helper helper RNAs were linearized with XhoI, before in vitro transcription using the mMESSAGE mMACHINE RNA transcription kit (SP6 version; Ambion). Both helper and replicon RNA transcripts (20 μl each) were then electroporated into BHK-21 cells and incubated in 10 ml of αMEM containing 5% FBS at 37° C. for 12 h. The medium was replaced with 9 ml of Opti-MEM I medium (GIBCO-BRL, Invitrogen San Diego Calif.) supplemented with 0.7 μM CaCl₂. After 24 h, the culture medium was collected and stored at −80° C.

DM-Helper Vector titering. The titers of Sindbis vectors were assayed in BHK-21 cells. Serial dilutions (50 μL each) of vector were added to 10⁴ BHK-21 cells in 96-well plates. After incubation for 1 hour at room temperature, the cells were washed with PBS and incubated with 200 μL of αMEM at 37° C. overnight (≈12 hours).

The next day total of RNA from each well was extracted separately using the Trizol extraction protocol (Invitrogen cat#15596-018). One μg of each RNA was used as template to make cDNA with primer cDNA5-R (5′-TTTTTGAAATGTTAAAAACAAAATTTTGTTG) and ThermoScript™ RNaseH-reverse transcriptase (Invitrogen cat#12236-022), at 60° C. for 2 h, following the manufacture S protocol.

The presence of Sindbis cDNA in each dilution was detected by real time PCR using the MyiQ Single-Color Real-Time PCR Detection System (BioRad, Cat#170-9740)

The PCR reactions were performed for each vector cDNA dilution. One hundred ng of the cDNA reaction were used as template for each PCR reaction, with 250 nM primers cDNA5-R (5′-TTTTTGAAATGTTAAAAACAAAATTTTGTTG) and 7692F (5′-TGATCCGACCAGCAAAACTC), using SGMIX (2×) (Biorad, Cat#170-882).

For generating the standard curve 10× serial dilutions of the XhoI linealized SP6RLacZ plasmid were used, three reactions per dilution, ranging from 10³ pg to 10⁻³ pg.

All reactions were performed in 96 well plates with thermic conditions: 95.0° C. 5 min, 40 cycles of: 95° C. 20 sec, 60.0° C. 30 sec and 72.0° C. 30 sec. Fluorescence data were acquired at 72.0° C. in each cycle. Melting curves started at 55.0° C. 10 see, increasing set-point temperature after cycle 2 by 0.5° C.

The presence of the 301 amplified band was checked by electrphoresis in agarose gels. The results of the titration are set forth in Table C.

Tables for Example 5

TABLE A Titer of Sindbis vectors in BHK cells Titer in VECTOR BHK A (JTBB/SP6RFluc) 10⁷ C (SP6-H/SP6Rfluc) 10⁵ Mut-1 (SP6H-K70/SP6RFluc) 10⁴ Mut-2 (SP6H-K70-V251/SP6RFluc) 10⁵ Mut-4 (SP6H-I3-K70-E181-V251/SP6RFluc) 10⁶ DM-Helper (DM-Helper/SP6R) 10⁹

TABLE B Primers to construct DM-Helper#9 Primer Sequence (5′→3′) RE TRNA-F GTGGGAG

GTACATTTAGGTGACACTATA AvrII GGATATAGTGGTGAGTATCCCCGCCTGTCATGCG MfeI GGAGACCGGGGTTCGGTTCCCCGACGGGGAGCCA AACAGCCGAC

CACTAC TRNA-R GTAGTG

GTCGGCTGTTTGGCTCCCCGTC MfeI GGGGAACCGAACCCCGGTCTCCCGCATGACAGGC AvrII GGGGATACTCACCACTATATCCTATAGTGTCACC TAAATGTAC

CTCCCAC T-F GTGGGAC

GTAC AvrII T-R GTAGTG

GTCGG MfeI AvrII-F GCTCACATGTGGGAG

GTACATTTAGGTG AvrII AC AvrII-R GTCACCTAAATGTAC

CTCCCACATGTGA GC E1-7275F GTCAGCCGGCCG

G

TGCAA SphI G BstZ17I E1-7275R CTTGCA

CCT

GCGGCCG BstZ17I GCTGAC SphI E1-237F CCGTACACGCAGGC

AGGATTTGAGATGT EarI GG E1-237R CCACATCTCAAATCCT

GCCTGCGTGTAC EarI GG

TABLE C Titer of DM-Helper vector in BHK cells by Real time PCR (M) (−) Band DILUTION DM-Empty (pg) AVERAGE Back in gel 1.E−06 4.70E+03 2.40E+03 3.55E+03 3.55E+03 + 1.E−07 5.56E−02 3.09E−02 4.33E−02 4.09E−02 + 1.E−08 1.42E−02 1.42E−02 1.18E−02 + 1.E−09 7.83E−02 6.55E−02 7.19E−02 6.95E−02 +  1E−10 4.57E−03 2.34E−03 3.46E−03 1.09E−03 −  1E−11 1.01E−02 5.82E−03 7.96E−03 5.59E−03 −  1E−12 1.68E−03 5.36E−03 3.52E−03 1.15E−03 − Water 1.32E−03 1.32E−03 −1.05E−03 − BHK-55 2.37E−03 2.37E−03 0.00E+00 −

TABLE D residue comparison of helper mutants Protein nt (a.a.) JT-BB Mut-4 DM-Helper 7846 Capsid (67) Pro Gln Gln 8638 E2 (3) Ile Ile Ile 8838 E2 (70) Lys Lys Lys 9171 E2 (181) Glu Glu Glu 9382 E2 (251) Val Val Val 10279 E1 (72) Ala Val Ala 10288 E1 (75) Gly Asp Gly 10773 E1 (237) Ser Ala Ser

TABLE E Comparison of 5¹ terminus of Helper plasmids in U.S. Pat. No. 5,091,309 and DM ′309 patent 1 GGATATAGTGGTGAGTATCCCCGCCTGTCACGCGGGAGACCGGGGTTCGGTTCCCCGACG 60 DM-Helper 1 ..............................T............................. 60 ′309 patent 61 GGGAGCCAAACAGCCGACCAATTGCACTACCATCACAATGGAGAAGCCAGTAGTAAACGT 120 DM-Helper 61 ............................................................ 120 ′309 patent 121 AGACGTAGACCCCCAGAGTCCGTTTGTCGTGCAACTGCAAAAA-GCTTCCCGCAATTTGA 179 DM-Helper 121 ...........................................A................ 180 ′309 patent 180 GGTAGTAGCACAGCAGGTCACTCCAAATGACCATGCTAATGCCAGAGCATTTTCGCATCT 239 DM-Helper 181 ............................................................ 240 ′309-patent 240 GGCCAGTAAACTAATCGAGCTGGAGGTTCCTACCACAGCGACGATCTTGGACATAGGCAG 299 DM-Helper 241 ............................................................ 300 ′309 patent 300 CGCACCGGCTCGTAGCATGTTTTCCGAGCACCAGTATCATTGTGTCTGCCCCATGCGTAG 359 DM-Helper 301 ...............A............................................ 360 ′309 patent 360 TCCAGAAGACCCGGACCGCATGATGAAATATGCCAGTAAACTGGCGGAAAAAGCGTGCAA 419 DM-Helper 361 ............................................................ 420 ′309 patent 420 GATTACAAACAAGAACTTGCATGAGAAGATTAAGGATCTCCGGA 463 DM-Helper 421 ............................................ 464 Score = 152 bits (79), Expect = 3e−33 Identities = 79/79 (100%), Gaps = 0/79 (0%) Strand = Plus/Plus ′309 Patent 526 AAATATGCCAGTAAACTGGCGGAAAAAGCGTGCAAGATTACAAACAAGAACTTGCATGAG 585 DM-Helper 386 ............................................................ 445 ′309 Patent 586 AAGATTAGGATCTCCGGA 604 DM-Helper 446 .................. 464 CPU time: 0.04 user secs. 0.02 sys. Secs 0.06 total secs.

TABLE F DM-Helper components Nt → nt Part from: Description 1_66 Oligos Nt 9 to nt 70 of Asp-tRNA sequence 67_464 SV nt 29_426 67_97 SV nt 29_59 5′ end untranscribed 98_464 SV nt 60_426 Nsp1 5′ end nt 1 to 367 461_4831 SV 7333_11703 SGP + Helper proteins 461_726 SV 7333_7598 Nt 1583 to nt 1848 of nsp4 sequence = 3′ end. Works as SGP enhancer 727_774 SV 7599_7646 Subgenomic promoter (SGP), core sequence 775_1566 SV 7647_8438 Capsid protein sequence 1567_1758 SV 8439_8630 E3 envelope protein sequence 1759_3027 SV 8631_9899 E2 envelope protein sequence 3028_3192 SV 9900_10064 6K peptide sequence 3193_4509 SV 10065_11381 E1 envelope protein sequence 4510_4830 SV 11382_11703 3′ end untranslated region 4831_4869 SV Poly A tail 4870_4897 Oligos Multi cloning site MCS 4898_6728 Bacterial part pUC 5034_5894 Bacterial part Beta Lactamase (=Amp resistant gene) pUC 6039_6668 Bacterial part Bacterial DNA replication origin ColE1 pUC 6729_6746 Oligos SP6 promoter

A restriction endonuclease map of the DM-Helper plasmid is shown in FIG. 14.

It can be seen from the data presented herein that defective Sindbis virus vectors which are produced using the novel DM-Helper plasmid disclosed herein have acquired the improved binding characteristics of the Mut-4 vector and production of higher titers of the JT vector.

DM-HELPER SEQUENCE (6746 bp) SEQ ID NO: 41 GGATATAGTGGTGAGTATCCCCGCCTGTCATGCGGGAGACCGGGGTTCGG TTCCCCGACGGGGAGCCAAACAGCCGACCAATTGCACTACCATCACAATG GAGAAGCCAGTAGTAAACGTAGACGTAGACCCCCAGAGTCCGTTTGTCGT GCAACTGCAAAAAAGCTTCCCGCAATTTGAGGTAGTAGCACAGCAGGTCA CTCCAAATGACCATGCTAATGCCAGAGCATTTTCGCATCTGGCCAGTAAA CTAATCGAGCTGGAGGTTCCTACCACAGCGACGATCTTGGACATAGGCAG CGCACCGGCTCGTAGAATGTTTTCCGAGCACCAGTATCATTGTGTCTGCC CCATGCGTAGTCCAGAAGACCCGGACCGCATGATGAAATATGCCAGTAAA CTGGCGGAAAAAGCGTGCAAGATTACAAACAAGAACTTGCATGAGAAGAT TAAGGATCTCCGGATCCCCTGAAAAGGCTGTTTAAGTTGGGTAAACCGCT CCCAGCCGACGACGAGCAAGACGAAGACAGAAGACGCGCTCTGCTAGATG AAACAAAGGCGTGGTTTAGAGTAGGTATAACAGGCACTTTAGCAGTGGCC GTGACGACCCGGTATGAGGTAGACAATATTACACCTGTCCTACTGGCATT GAGAACTTTTGCCCAGAGCAAAAGAGCATTCCAAGCCATCAGAGGGGAAA TAAAGCATCTCTACGGTGGTCCTAAATAGTCAGCATAGTACATTTCATCT GACTAATACTACAACACCACCACCATGAATAGAGGATTCTTTAACATGCT CGGCCGCCGCCCCTTCCCGGCCCCCACTGCCATGTGGAGGCCGCGGAGAA GGAGGCAGGCGGCCCCGATGCCTGCCCGCAACGGGCTGGCTTCTCAAATC CAGCAACTGACCACAGCCGTCAGTGCCCTAGTCATTGGACAGGCAACTAG ACCTCAACCCCCACGTCCACGCCAGCCACCGCGCCAGAAGAAGCAGGCGC CCAAGCAACCACCGAAGCCGAAGAAACCAAAAACGCAGGAGAAGAAGAAG AAGCAACCTGCAAAACCCAAACCCGGAAAGAGACAGCGCATGGCACTTAA GTTGGAGGCCGACAGATTGTTCGACGTCAAGAACGAGGACGGAGATGTCA TCGGGCACGCACTGGCCATGGAAGGAAAGGTAATGAAACCTCTGCACGTG AAAGGAACCATCGACCACCCTGTGCTATCAAAGCTCAAATTTACCAAGTC GTCAGCATACGACATGGAGTTCGCACAGTTGCCAGTCAACATGAGAAGTG AGGCATTCACCTACACCAGTGAACACCCCGAAGGATTCTATAACTGGCAC CACGGAGCGGTGCAGTATAGTGGAGGTAGATTTACCATCCCTCGCGGAGT AGGAGGCAGAGGAGACAGCGGTCGTCCGATCATGGATAACTCCGGTCGGG TTGTCGCGATAGTCCTCGGTGGAGCTGATGAAGGAACACGAACTGCCCTT TCGGTCGTCACCTGGAATAGTAAAGGGAAGACAATTAAGACGACCCCGGA AGGGACAGAAGAGTGGTCCGCAGCACCACTGGTCACGGCAATGTGTTTGC TCGGAAATGTGAGCTTCCCATGCGACCGCCCGCCCACATGCTATACCCGC GAACCTTCCAGAGCCCTCGACATCCTTGAAGAGAACGTGAACCATGAGGC CTACGATACCCTGCTCAATGCCATATTGCGGTGCGGATCGTCTGGCAGAA GCAAAAGAAGCGTCATCGATGACTTTACCCTGACCAGCCCCTACTTGGGC ACATGCTCGTACTGCCACCATACTGAACCGTGCTTCAGCCCTGTTAAGAT CGAGCAGGTCTGGGACGAAGCGGACGATAACACCATACGCATACAGACTT CCGCCCAGTTTGGATACGACCAAAGCGGAGCAGCAAGCGCAAACAAGTAC CGCTACATGTCGCTTAAGCAGGATCACACCGTTAAAGAAGGCACCATGGA TGACATCAAGATTAGCACCTCAGGACCGTGTAGAAGGCTTAGCTACAAAG GATACTTTCTCCTCGCAAAATGCCCTCCAGGGGACAGCGTAACGGTTAGC ATAGTGAGTAGCAACTCAGCAACGTCATGTACACTGGCCCGCAAGATAAA ACCAAAATTCGTGGGACGGGAAAAATATGATCTACCTCCCGTTCACGGTA AAAAAATTCCTTGCACAGTGTACGACCGTCTGAAAGAAACAACTGCAGGC TACATCACTATGCACAGGCCGGGCCCGCACGCTTATACATCCTACCTGGA AGAATCATCAGGGAAAGTTTACGCAAAGCCGCCATCTGGGAAGAACATTA CGTATGAGTGCAAGTGCGGCGACTACAAGACCGGAACCGTTTCGACCCGC ACCGAAATCACTGGTTGCACCGCCATCAAGCAGTGCGTCGCCTATAAGAG CGACCAAACGAAGTGGGTCTTCAACTCACCGGACTTGATCCGACATGACG ACCACACGGTCCAAGGGAAATTGCATTTGCCTTTCAAGTTGATCCCGAGT ACCTGCATGGTCCCTGTTGCCCACGCGCCGAATGTAATACATGGCTTTAA ACACATCAGCCTCCAATTAGATACAGACCACTTGACATTGCTCACCACCA GGAGACTAGGGGCAAACCCGGAACCAACCACTGAATGGATCGTCGGAAAG ACGGTCAGAAACTTCACCGTCGACCGAGATGGCCTGGAATACATATGGGG AAATCATGAGCCAGTGAGGGTCTATGCCCAAGAGTCAGCACCAGGAGACC CTCACGGATGGCCACACGAAATAGTACAGCATTACTACCATCGCCATCCT GTGTACACCATCTTAGCCGTCGCATCAGCTACCGTGGCGATGATGATTGG CGTAACTGTTGCAGTGTTATGTGCCTGTAAAGCGCGCCGTGAGTGCCTGA CGCCATACGCCCTGGCCCCAAACGCCGTAATCCCAACTTCGCTGGCACTC TTGTGCTGCGTTAGGTCGGCCAATGCTGAAACGTTCACCGAGACCATGAG TTACTTGTGGTCGAACAGTCAGCCGTTCTTCTGGGTCCAGTTGTGCATAC CTTTGGCCGCTTTCATCGTTCTAATGCGCTGCTGCTCCTGCTGCCTGCCT TTTTTAGTGGTTGCCGGCGCCTACCTGGCGAAGGTAGACGCCTACGAACA TGCGACCACTGTTCCAAATGTGCCACAGATACCGTATAAGGCACTTGTTG AAAGGGCAGGGTATGCCCCGCTCAATTTGGAGATCACTGTCATGTCCTCG GAGGTTTTGCCTTCCACCAACCAAGAGTACATTACCTGCAAATTCACCAC TGTGGTCCCCTCCCCAAAAATCAAATGCTGCGGCTCCTTGGAATGTCAGC CGGCCGCGCATGCAGGGTATACCTGCAAGGTCTTCGGAGGGGTCTACCCC TTTATGTGGGGAGGAGCGCAATGTTTTTGCGACAGTGAGAACAGCCAGAT GAGTGAGGCGTACGTCGAACTGTCAGCAGATTGCGCGTCTGACCACGCGC AGGCGATTAAGGTGCACACTGCCGCGATGAAAGTAGGACTGCGTATAGTG TACGGGAACACTACCAGTTTCCTAGATGTGTACGTGAACGGAGTCACACC AGGAACGTCTAAAGACTTGAAAGTCATAGCTGGACCAATTTCAGCATCGT TTACGCCATTCGATCATAAGGTCGTTATCCATCGCGGCCTGGTGTACAAC TATGACTTCCCGGAATATGGAGCGATGAAACCAGGAGCGTTTGGAGACAT TCAAGCTACCTCCTTGACTAGCAAGGATCTCATCGCCAGCACAGACATTA GGCTACTCAAGCCTTCCGCCAAGAACGTGCATGTCCCGTACACGCAGGCC TCTTCAGGATTTGAGATGTGGAAAAACAACTCAGGCCGCCCACTGCAGGA AACCGCACCTTTCGGGTGTAAGATTGCAGTAAATCCGCTCCGAGCGGTGG ACTGTTCATACGGGAACATTCCCATTTCTATTGACATCCCGAACGCTGCC TTTATCAGGACATCAGATGCACCACTGGTCTCAACAGTCAAATGTGAAGT CAGTGAGTGCACTTATTCAGCAGACTTCGGCGGGATGGCCACCCTGCAGT ATGTATCCGACCGCGAAGGTCAATGCCCCGTACATTCGCATTCGAGCACA GCAACTCTCCAAGAGTCGACAGTACATGTCCTGGAGAAAGGAGCGGTGAC AGTACACTTTAGCACCGCGAGTCCACAGGCGAACTTTATCGTATCGCTGT GTGGGAAGAAGACAACATGCAATGCAGAATGTAAACCACCAGCTGACCAT ATCGTGAGCACCCCGCACAAAAATGACCAAGAATTTCAAGCCGCCATCTC AAAAACATCATGGAGTTGGCTGTTTGCCCTTTTCGGCGGCGCCTCGTCGC TATTAATTATAGGACTTATGATTTTTGCTTGCAGCATGATGCTGACTAGC ACACGAAGATGACCGCTACGCCCCAATGATCCGACCAGCAAAACTCGATG TACTTCCGAGGAACTGATGTGCATAATGCATCAGGCTGGTACATTAGATC CCCGCTTACCGCGGGCAATATAGCAACACTAAAAACTCGATGTACTTCCG AGGAAGCGCAGTGCATAATGCTGCGCAGTGTTGCCACATAACCACTATAT TAACCATTTATCTAGCGGACGCCAAAAACTCAATGTATTTCTGAGGAAGC GTGGTGCATAATGCCACGCAGCGTCTGCATAACTTTTATTATTTCTTTTA TTAATCAACAAAATTTTGTTTTTAACATTTCAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAATTTAAATTAATTAAGCGGCCGCCTCGAGGAC GTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTAT TTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGA TAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTT CCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTG CTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGT GCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGA GAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTC TGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTC GGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGT CACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTG CTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACG ATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCA TGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAA ACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGC AAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAAT AGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCC TTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGG TCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTAT CGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATA GACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCA GACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTA ATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAA TCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAG ATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTT GCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAG AGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATA CCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAA CTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGG CTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGA TAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCAC ACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGC GTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGG TATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCC AGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCT GACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGG AAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCC TTTTGCTCACATGTGGGAGCCTAGGGTACATTTAGGTGACACTATA

REFERENCES FOR EXAMPLE 5

-   20.—Schlesinger, S., R. Levis, B. G. Weiss, M. Tsiang, and H.     Huang. 1987. Replication and packaging sequences in defective     interfering RNAs of Sindbis virus, p. 241-250. In M. A. Brinton     and R. R. Rueckert (cd.), Positive strand RNA viruses. Alan R. Liss,     New York. -   21 Sindbis virus expression vectors: packaging of RNA replicons by     using defective helper RNAs. Bredenbeck P J, Frolov I, Rice C M,     Schlesinger S. J Virol. 1993 November; 67(11):6439-46.

Example 6 Generation of Plasmid T7DM-Helper#101

The T7DM-Helper#101 plasmid is derived from the DM-helper plasmid described above in Example 5. The Sindbis sequence that is transcribed in vitro from this plasmid to make the RNA for electroporation is the same as the DMHelper. The difference is that the SP6 promoter was replaced by the T7 promoter and two EcoRV restriction sites were added flanking the T7-Sindbis sequence, to allow for subcloning this fragments in other vectors.

Summary of Cloning Strategy

Step 1: Oligos were generated from the sequence: AvrII-EcoRV-T7-Sindbis5end-MfeI and clone in the TOPO vector. Plasmid T1

Step 2: The EcoRV restriction site was inserted in the 3′ end of the Sindbis sequence in the DMHelper plasmid, after the XhoI site used to linearize the DNA for in vitro transcription. Plasmid EcoRV_DMHelper#6

Step 3: The AvrII-5end-MfeI fragment was cloned into the plasmid generated in step 2. Plasmid T7DM-Helper#101.

T7DM-Helper#101 Cloning

Plasmid T1.

The reaction mix of primers T7-5End-F and T7-5End-R (4.4 ug/each) in water, was boiled for 1 min and cooled down to RT for primer annealing.

One hundred ng of the primer mix was ligated to the pcr4blunt_topo vector (Invitrogen, San Diego, Calif.) to make the new plasmid T1. The oligo insert was sequenced.

Plasmid EcoRV_DMHelper#6

The EcoRV_DMHelper#6 helper mas made from plasmid DM-Helper is using the QuickChange IIx site-directed mutagenesis kit following the manufacturer's instructions (Stratagene, La Jolla, Calif.). Briefly, 20 ng of DM-Helper were incubated with complementary primers: EcoRV-End-F/EcoRV-End-R, reaction buffer, dNTPs and 2.5 units of pfu Ultra HF DNA polymerase. PCR reactions were 95° C. 1 min, 18 cycles of 95° C. 50 s, 60° C. 50 s, 68° C. 13.5 min, and final elongation of 68° C. 7 min.

After the PCR reactions, 10 U of restriction enzyme DpnI was added and the reaction was incubated at 37° C. for 2 hours to digest the methylated parental DNA. XL-10-Gold competent E. coli cells were transformed and the colonies analyzed by restriction EcoRV enzyme digestion. Mutations in the new plasmid were verified by sequencing.

Plasmid T7DM-Helper#101.

The T1 Plasmid was digested with enzymes AvrII and MfeI O/N at 37° C. The 130 by band was isolated from an agarose gel and ligated in the AvrII and MfeI digested EcoRV_DMHelper#6 plasmid. E. coli DH5-α cells were transformed with the ligation and colonies were screened by AvrII/MfeI digestion. The new plasmid, T7DM-Helper#101, was fully sequenced.

TABLE 1 PRIMER SEQUENCE RE T7-5End-F

AvrII EcoRV Mfel T7-5End-R GTGCAATTGGTCGGCTGTTTGGCTCCCCGTCGGGGAACCGAACCC CGGTCTCCCGCATGACAGGCGGGGATACTCACCACTATATCCTAT AGTGAGTCGTATTAAATTCGTAATCATGTCATAGCTGTTGATATC CTAGGATC EcoRV-End-F GCGGCCGCCTCGAGGACGGATATCGCACTTTTCGGGGAAA Xhol EcoRV EcoRV-End-R TTTCCCCGAAAAGTGCGATATCACGTCCTCGAGGCGGCCGC EcoRV Xhol primers used to generate T7DM-Helper #101 vector. Shaded T7 promote sequence

Plasmid T7DM-Helper#101 sequece (6771 bp): GGATATAGTGGTGAGTATCCCCGCCTGTCATGCGGGAGACCGGGGTTCGG TTCCCCGACGGGGAGCCAAACAGCCGACCAATTGCACTACCATCACAATG GAGAAGCCAGTAGTAAACGTAGACGTAGACCCCCAGAGTCCGTTTGTCGT GCAACTGCAAAAAAGCTTCCCGCAATTTGAGGTAGTAGCACAGCAGGTCA CTCCAAATGACCATGCTAATGCCAGAGCATTTTCGCATCTGGCCAGTAAA CTAATCGAGCTGGAGGTTCCTACCACAGCGACGATCTTGGACATAGGCAG CGCACCGGCTCGTAGAATGTTTTCCGAGCACCAGTATCATTGTGTCTGCC CCATGCGTAGTCCAGAAGACCCGGACCGCATGATGAAATATGCCAGTAAA CTGGCGGAAAAAGCGTGCAAGATTACAAACAAGAACTTGCATGAGAAGAT TAAGGATCTCCGGATCCCCTGAAAAGGCTGTTTAAGTTGGGTAAACCGCT CCCAGCCGACGACGAGCAAGACGAAGACAGAAGACGCGCTCTGCTAGATG AAACAAAGGCGTGGTTTAGAGTAGGTATAACAGGCACTTTAGCAGTGGCC GTGACGACCCGGTATGAGGTAGACAATATTACACCTGTCCTACTGGCATT GAGAACTTTTGCCCAGAGCAAAAGAGCATTCCAAGCCATCAGAGGGGAAA TAAAGCATCTCTACGGTGGTCCTAAATAGTCAGCATAGTACATTTCATCT GACTAATACTACAACACCACCACCATGAATAGAGGATTCTTTAACATGCT CGGCCGCCGCCCCTTCCCGGCCCCCACTGCCATGTGGAGGCCGCGGAGAA GGAGGCAGGCGGCCCCGATGCCTGCCCGCAACGGGCTGGCTTCTCAAATC CAGCAACTGACCACAGCCGTCAGTGCCCTAGTCATTGGACAGGCAACTAG ACCTCAACCCCCACGTCCACGCCAGCCACCGCGCCAGAAGAAGCAGGCGC CCAAGCAACCACCGAAGCCGAAGAAACCAAAAACGCAGGAGAAGAAGAAG AAGCAACCTGCAAAACCCAAACCCGGAAAGAGACAGCGCATGGCACTTAA GTTGGAGGCCGACAGATTGTTCGACGTCAAGAACGAGGACGGAGATGTCA TCGGGCACGCACTGGCCATGGAAGGAAAGGTAATGAAACCTCTGCACGTG AAAGGAACCATCGACCACCCTGTGCTATCAAAGCTCAAATTTACCAAGTC GTCAGCATACGACATGGAGTTCGCACAGTTGCCAGTCAACATGAGAAGTG AGGCATTCACCTACACCAGTGAACACCCCGAAGGATTCTATAACTGGCAC CACGGAGCGGTGCAGTATAGTGGAGGTAGATTTACCATCCCTCGCGGAGT AGGAGGCAGAGGAGACAGCGGTCGTCCGATCATGGATAACTCCGGTCGGG TTGTCGCGATAGTCCTCGGTGGAGCTGATGAAGGAACACGAACTGCCCTT TCGGTCGTCACCTGGAATAGTAAAGGGAAGACAATTAAGACGACCCCGGA AGGGACAGAAGAGTGGTCCGCAGCACCACTGGTCACGGCAATGTGTTTGC TCGGAAATGTGAGCTTCCCATGCGACCGCCCGCCCACATGCTATACCCGC GAACCTTCCAGAGCCCTCGACATCCTTGAAGAGAACGTGAACCATGAGGC CTACGATACCCTGCTCAATGCCATATTGCGGTGCGGATCGTCTGGCAGAA GCAAAAGAAGCGTCATCGATGACTTTACCCTGACCAGCCCCTACTTGGGC ACATGCTCGTACTGCCACCATACTGAACCGTGCTTCAGCCCTGTTAAGAT CGAGCAGGTCTGGGACGAAGCGGACGATAACACCATACGCATACAGACTT CCGCCCAGTTTGGATACGACCAAAGCGGAGCAGCAAGCGCAAACAAGTAC CGCTACATGTCGCTTAAGCAGGATCACACCGTTAAAGAAGGCACCATGGA TGACATCAAGATTAGCACCTCAGGACCGTGTAGAAGGCTTAGCTACAAAG GATACTTTCTCCTCGCAAAATGCCCTCCAGGGGACAGCGTAACGGTTAGC ATAGTGAGTAGCAACTCAGCAACGTCATGTACACTGGCCCGCAAGATAAA ACCAAAATTCGTGGGACGGGAAAAATATGATCTACCTCCCGTTCACGGTA AAAAAATTCCTTGCACAGTGTACGACCGTCTGAAAGAAACAACTGCAGGC TACATCACTATGCACAGGCCGGGCCCGCACGCTTATACATCCTACCTGGA AGAATCATCAGGGAAAGTTTACGCAAAGCCGCCATCTGGGAAGAACATTA CGTATGAGTGCAAGTGCGGCGACTACAAGACCGGAACGGTTTCGACCCGC ACCGAAATCACTGGTTGCACCGCCATCAAGCAGTGCGTCGCCTATAAGAG CGACCAAACGAAGTGGGTCTTCAACTCACCGGACTTGATCCGACATGACG ACCACACGGTCCAAGGGAAATTGCATTTGCCTTTCAAGTTGATCCCGAGT ACCTGCATGGTCCCTGTTGCCCACGCGCCGAATGTAATACATGGCTTTAA ACACATCAGCCTCCAATTAGATACAGACCACTTGACATTGCTCACCACCA GGAGACTAGGGGCAAACCCGGAACCAACCACTGAATGGATCGTCGGAAAG ACGGTCAGAAACTTCACCGTCGACCGAGATGGCCTGGAATACATATGGGG AAATCATGAGCCAGTGAGGGTCTATGCCCAAGAGTCAGCACCAGGAGACC CTCACGGATGGCCACACGAAATAGTACAGCATTACTACCATCGCCATCCT GTGTACACCATCTTAGCCGTCGCATCAGCTACCGTGGCGATGATGATTGG CGTAACTGTTGCAGTGTTATGTGCCTGTAAAGCGCGCCGTGAGTGCCTGA CGCCATACGCCCTGGCCCCAAACGCCGTAATCCCAACTTCGCTGGCACTC TTGTGCTGCGTTAGGTCGGCCAATGCTGAAACGTTCACCGAGACCATGAG TTACTTGTGGTCGAACAGTCAGCCGTTCTTCTGGGTCCAGTTGTGCATAC CTTTGGCCGCTTTCATCGTTCTAATGCGCTGCTGCTCCTGCTGCCTGCCT TTTTTAGTGGTTGCCGGCGCCTACCTGGCGAAGGTAGACGCCTACGAACA TGCGACCACTGTTCCAAATGTGCCACAGATAGCGTATAAGGCACTTGTTG AAAGGGCAGGGTATGCCCCGCTCAATTTGGAGATCACTGTCATGTGCTCG GAGGTTTTGCCTTCCACCAACCAAGAGTACATTACCTGCAAATTCACCAC TGTGGTCCCCTCCCCAAAAATCAAATGCTGCGGCTCCTTGGAATGTCAGC CGGCCGCGCATGCAGGGTATACCTGCAAGGTCTTCGGAGGGGTCTACCCC TTTATGTGGGGAGGAGCGCAATGTTTTTGCGACAGTGAGAACAGCCAGAT GAGTGAGGCGTACGTCGAACTGTCAGCAGATTGCGCGTCTGACCACGCGC AGGCGATTAAGGTGCACACTGCCGCGATGAAAGTAGGACTGGGTATAGTG TACGGGAACACTACCAGTTTCCTAGATGTGTACGTGAACGGAGTCACACC AGGAACGTCTAAAGACTTGAAAGTCATAGCTGGACCAATTTCAGCATCGT TTACGCCATTCGATCATAAGGTCGTTATCCATCGCGGCCTGGTGTACAAC TATGACTTCCCGGAATATGGAGCGATGAAACCAGGAGCGTTTGGAGACAT TCAAGCTACCTCCTTGACTAGCAAGGATCTCATCGCCAGCACAGACATTA GGCTACTCAAGCCTTCCGCCAAGAACGTGCATGTCCCGTACACGCAGGCC TCTTCAGGATTTGAGATGTGGAAAAACAACTCAGGCCGCCCACTGCAGGA AACCGCACCTTTCGGGTGTAAGATTGCAGTAAATCCGCTCCGAGCGGTGG ACTGTTCATACGGGAACATTCCCATTTCTATTGACATCCCGAACGCTGCC TTTATCAGGACATCAGATGCACCACTGGTCTCAACAGTCAAATGTGAAGT CAGTGAGTGCACTTATTCAGCAGACTTCGGCGGGATGGCCACCCTGCAGT ATGTATCCGACCGCGAAGGTCAATGCCCCGTACATTCGCATTCGAGCACA GCAACTCTCCAAGAGTCGACACTACATGTCCTGGAGAAAGGAGCGGTGAC AGTACACTTTAGCACCGCGAGTCCACAGGCGAACTTTATCGTATCGCTGT GTGGGAAGAAGACAACATGCAATGCAGAATGTAAACCACCAGCTGACCAT ATCGTGAGCACCCCGCACAAAAATGACCAAGAATTTCAAGCCGCCATCTC AAAAACATCATGGAGTTGGCTGTTTGCCCTTTTCGGCGGCGCCTCGTCGC TATTAATTATAGGACTTATGATTTTTGCTTGCAGCATGATGCTGACTAGC ACACGAAGATGACCGCTACGCCCCAATGATCCGACCAGCAAAACTCGATG TACTTCCGAGGAACTGATGTGCATAATGCATCAGGCTGGTACATTAGATC CCCGCTTACCGCGGGCAATATAGCAACACTAAAAACTCGATGTACTTCCG AGGAAGCGCAGTGCATAATGCTGCGCAGTGTTGCCACATAACCACTATAT TAACCATTTATCTAGCGGACGCCAAAAACTCAATGTATTTCTGAGGAAGC GTGGTGCATAATGCCACGCAGCGTCTGCATAACTTTTATTATTTCTTTTA TTAATCAACAAAATTTTGTTTTTAACATTTCAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAATTTAAATTAATTAAGCGGCCGCCTCGAGGACG TGATATCGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATT TTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGAT AAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTGAACATTTC CGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGC TCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTG CACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAG AGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCT GCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCG GTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTC ACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGC TGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGA TCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCAT GTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAA CGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCA AACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATA GACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCT TCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGT CTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATC GTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAG ACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAG ACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAA TTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAAT CCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGA TCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTG CAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGA GCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATAC CAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAAC TCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGC TGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGAT AGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACA CAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCG TGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGT ATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCA GGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTG ACTTGAGCGTCGATTTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGG AAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCC TTTTGCTCACATGTGGGAGCCTAGGATATCAACAGCTATGACATGATTAC GAATTTAATACGACTCACTATA

Example 7 Generation of Plasmid T7StuI-RLacZ#22

The T7StuI-RLacZ#22 plasmid is derived from the T7-RLacZ#202 plasmid. The Sindbis sequence that is transcribed in vitro from this plasmid to make the RNA for electroporation is the same as for T7-RLacZ#202. Two StuI restriction sites were added flanking the T7-Sindbis sequence, to allow for subcloning this fragments in other vectors.

The double mutant T7-StuI-RLacZ#202 plasmid was made by mutagenesis of plasmid T7-RLacZ#202 in two steps: first by introducing a StuI Restriction site in the 5′ end to make the 5-StuIRLacZ#2 plasmid, and second using this new vector to mutagenize the 3′ end.

Double mutations were made on plasmid T7-StuI-RLacZ#202 using the QuickChange IIx site-directed mutagenesis kit by following the manufacturer's instructions (Stratagene, La Jolla, Calif.). Briefly, 20 ng of T7-StuI-RLacZ#202 were incubated with complementary primers: 5-T7LZ-StuIF/5-T7LZ-StuIR, reaction buffer, dNTPs and 2.5 units of pfu Ultra HF DNA polymerase. PCR reactions were 95° C. 1 min, 18 cycles of: 95° C. 50 s, 60° C. 50 s, 68° C. 13.5 min, and final elongation of 68° C. 7 min.

After the PCR reactions, 10 U of restriction enzyme DpnI was added and the reaction was incubated at 37° C. for 1 hour to digest the methylated parental DNA. XL-10-Gold competent E. coli cells were transformed and the colonies analyzed by restriction enzyme digestion (RE). Mutations in the new plasmid were verified by sequencing.

The final plasmid (T7StuI-RLacZ#22) was made following the same protocol using the new plasmid 5-StuIRLacZ#2 as a template for PCR and primers 3-T7LZ-StuI-F/3-T7LZ-StuI-R.

The sequence of primers used and nt changes are shown in Table 2

TABLE 2 Underline RE sequence. Low case residues mutated PRIMER NAME SEQUENCE RE SITE 5-T7LZ-StuIF CATGTGGG

GAGTACTTAATACGACTCACTATAGG StuI 5-T7LZ-StuIR CCTATAGTGAGTCGTATTAAGTACTC

CCCACATG StuI 3-T7LZ-StuIF CCTCGAGGACGTCAGGT

TTTCGGGGAAATGTGC StuI 3-T7LZ-StuIR GCACATTTCCCCGAAA

ACCTGACGTCCTCGAGG StuI

Plasmid T7StuI-RLacZ#22 sequence (13092 bp) ATTGACGGCGTAGTACACACTATTGAATCAAACAGCCGACCAATTGCACT ACCATCACAATGGAGAAGCCAGTAGTAAACGTAGACGTAGACCCCCAGAG TCCGTTTGTCGTGCAACTGCAAAAAAGCTTCCCGCAATTTGAGGTAGTAG CACAGCAGGTCACTCCAAATGACCATGCTAATGCCAGAGCATTTTCGCAT CTGGCCAGTAAACTAATCGAGCTGGAGGTTCCTACCACAGCGACGATCTT GGACATAGGCAGCGCACCGGCTCGTAGAATGTTTTCCGAGCACCAGTATC ATTGTGTCTGCCCCATGCGTAGTCCAGAAGACCCGGACCGCATGATGAAA TATGCCAGTAAACTGGCGGAAAAAGCGTGCAAGATTACAAACAAGAACTT GCATGAGAAGATTAAGGATCTCCGGACCGTACTTGATACGCCGGATGCTG AAACACCATCGCTCTGCTTTCACAACGATGTTACCTGCAACATGCGTGCC GAATATTCCGTCATGCAGGACGTGTATATCAACGCTCCCGGAACTATCTA TCATCAGGCTATGAAAGGCGTGCGGACCCTGTACTGGATTGGCTTCGACA CCACCCAGTTCATGTTCTCGGCTATGGCAGGTTCGTACCCTGCGTACAAC ACCAACTGGGCCGACGAGAAAGTCCTTGAAGCGCGTAACATCGGACTTTG CAGCACAAAGCTGAGTGAAGGTAGGACAGGAAAATTGTCGATAATGAGGA AGAAGGAGTTGAAGCCCGGGTCGCGGGTTTATTTCTCCGTAGGATCGACA CTTTATCCAGAACACAGAGCCAGCTTGCAGAGCTGGCATCTTCCATCGGT GTTCCACTTGAATGGAAAGCAGTCGTACACTTGCCGCTGTGATACAGTGG TGAGTTGCGAAGGCTACGTAGTGAAGAAAATCACCATCAGTCCCGGGATC ACGGGAGAAACCGTGGGATACGCGGTTACACACAATAGCGAGGGCTTCTT GCTATGCAAAGTTACTGACACAGTAAAAGGAGAACGGGTATCGTTCCCTG TGTGCACGTACATCCCGGCCACCATATGCGATCAGATGACTGGTATAATG GCCACGGATATATCACCTGACGATGCACAAAAACTTCTGGTTGGGCTCAA CCAGCGAATTGTCATTAACGGTAGGACTAACAGGAACACCAACACCATGC AAAATTACCTTCTGCCGATCATAGCACAAGGGTTCAGCAAATGGGCTAAG GAGCGCAAGGATGATCTTGATAACGAGAAAATGCTGGGTACTAGAGAACG CAAGCTTACGTATGGCTGCTTGTGGGCGTTTCGCACTAAGAAAGTACATT CGTTTTATCGCCCACCTGGAACGCAGACCATCGTAAAAGTCCCAGCCTCT TTTAGCGCTTTTCCCATGTCGTCCGTATGGACGACCTCTTTGCCCATGTC GCTGAGGCAGAAATTGAAACTGGCATTGCAACCAAAGAAGGAGGAAAAAC TGCTGCAGGTCTCGGAGGAATTAGTCATGGAGGCCAAGGCTGCTTTGGAG GATGCTCAGGAGGAAGCCAGAGCGGAGAAGCTCCGAGAAGCACTTCCACC ATTAGTGGCAGACAAAGGCATCGAGGCAGCCGCAGAAGTTGTCTGCGAAG TGGAGGGGCTCCAGGCGGACATCGGAGCAGCATTAGTTGAAACCCCGCGC GGTCACGTAAGGATAATACCTCAAGCAAATGACCGTATGATCGGACAGTA TATCGTTGTCTCGCCAAACTCTGTGCTGAAGAATGCCAAACTCGGACCAG CGCACCCGCTAGCAGATCAGGTTAAGATCATAACACACTCCGGAAGATCA GGAAGGTACGCGGTCGAACCATACGACGCTAAAGTACTGATGCCAGCAGG AGGTGCCGTACCATGGCCAGAATTCCTAGCACTGAGTGAGAGCGCCACGT TAGTGTACAACGAAAGAGAGTTTGTGAACGGCAAACTATACCACATTGCC ATGCATGGCCCCGCCAAGAATACAGAAGAGGAGCAGTACAAGGTTACAAA GGCAGAGCTTGCAGAAACAGAGTACGTGTTTGACGTGGACAAGAAGCGTT GCGTTAAGAAGGAAGAAGCCTCAGGTCTGGTCCTCTCGGGAGAACTGACC AACCCTCCCTATCATGAGCTAGCTCTGGAGGGACTGAAGACCCGACCTGC GGTCCCGTACAAGGTCGAAACAATAGGAGTGATAGGCACACCGGGGTCGG GCAAGTCAGCTATTATCAAGTCAACTGTCACGGCACGAGATCTTGTTACC AGCGGAAAGAAAGAAAATTGTCGCGAAATTGAGCCCGACGTGCTAAGACT GAGGGGTATGCAGATTACGTCGAAGACAGTAGATTCGGTTATGCTCAACG GATGCCACAAAGCCGTAGAAGTGCTGTACGTTGACGAAGCGTTCGCGTGC CACGCAGGAGCACTACTTGCCTTGATTGCTATCGTCAGGCCCCGCAAGAA GGTAGTACTATGCGGAGACCCCATGCAATGCGGATTCTTCAACATGATGC AACTAAAGGTACATTTCAATCACCCTGAAAAAGACATATGCACCAAGACA TTCTACAAGTATATCTCCCGGCGTTGCACACAGCCAGTTACAGCTATTGT ATCGACACTGCATTACGATGGAAAGATGAAAACCACGAACCCGTGCAAGA AGAACATTGAAATCGATATTACAGGGGCCACAAAGCCGAAGCCAGGGGAT ATCATCCTGACATGTTTCCGCGGGTGGGTTAAGCAATTGCAAATCGACTA TCCCGGACATGAAGTAATGACAGCCGCGGCCTCACAAGGGCTAACCAGAA AAGGAGTGTATGCCGTCCGGCAAAAAGTCAATGAAAACCCACTGTACGCG ATCACATCAGAGCATGTGAACGTGTTGCTCACCCGCACTGAGGACAGGCT AGTGTGGAAAACC1TGCAGGGCGACCCATGGATTAAGCAGCTCACTAACA TACCTAAAGGAAACTTTCAGGCTACTATAGAGGACTGGGAAGCTGAACAC AAGGGAATAATTGCTGCAATAAACAGCCCCACTCCCCGTGCCAATCCGTT CAGCTGCAAGACCAACGTTTGCTGGGCGAAAGCATTGGAACCGATACTAG CCACGGCCGGTATCGTACTTACCGGTTGCCAGTGGAGCGAACTGTTCCCA CAGTTTGCGGATGACAAACCACATTCGGCCATTTACGCCTTAGACGTAAT TTGCATTAAGTTTTTCGGCATGGACTTGACAAGCGGACTGTTTTCTAAAC AGAGCATCCCACTAACGTACCATCCCGCCGATTCAGCGAGGCCGGTAGCT CATTGGGACAACAGCCCAGGAACCCGCAAGTATGGGTACGATCACGCCAT TGCCGCCGAACTCTCCCGTAGATTTCCGGTGTTCCAGCTAGCTGGGAAGG GCACACAACTTGATTTGCAGACGGGGAGAACCAGAGTTATCTCTGCACAG CATAACCTGGTCCCGGTGAACCGCAATCTTCCTCACGCCTTAGTCCCCGA GTACAAGGAGAAGCAACCCGGCCCGGTCGAAAAATTCTTGAACGAGTTCA AAGACCACTCAGTACTTGTGGTATCAGAGGAAAAAATTGAAGCTCCCCGT AAGAGAATCGAATGGATCGCCCCGATTGGCATAGCCGGTGCAGATAAGAA CTACAACCTGGCTTTCGGGTTTCCGCCGCAGGCACGGTACGACCTGGTGT TCATCAACATTGGAACTAAATACAGAAACCACCACTTTCAGCAGTGCGAA GACCATGCGGCGACCTTAAAAACCCTTTCGCGTTCGGCCCTGAATTGCCT TAACCCAGGAGGCACCCTCGTGGTGAAGTCCTATGGCTACGCCGACCGCA ACAGTGAGGACGTAGTCACCGCTCTTGCCAGAAAGTTTGTCAGGGTGTCT GCAGCGAGACCAGATTGTGTCTCAAGCAATACAGAAATGTACCTGATTTT CCGACAACTAGACAACAGCCGTACACGGCAATTCACCCCGCACCATCTGA ATTGCGTGATTTCGTCCGTGTATGAGGGTACAAGAGATGGAGTTGGAGCC GCGCCGTCATACCGCACCAAAAGGGAGAATATTGCTGACTGTCAAGAGGA AGCAGTTGTCAACGCAGCCAATCCGCTGGGTAGACCAGGCGAAGGAGTCT GCCGTGCCATCTATAAACGTTGGCCGACCAGTTTTACCGATTCAGCCACG GAGACAGGCACCGCAAGAATGACTGTGTGCCTAGGAAAGAAAGTGATCCA CGCGGTCGGCCCTGATTTCCGGAAGCACCCAGAAGCAGAAGCCTTGAAAT TGCTACAAAACGCCTACCATGCAGTGGCAGACTTAGTAAATGAACATAAC ATCAAGTCTGTCGCCATTCCACTGCTATCTACAGGCATTTACGCAGCCGG AAAAGACCGCCTTGAAGTATCACTTAACTGCTTGACAACCGCGCTAGACA GAACTGACGCGGACGTAACCATCTATTGCCTGGATAAGAAGTGGAAGGAA AGAATCGACGCGGCACTCCAACTTAAGGAGTCTGTAACAGAGCTGAAGGA TGAAGATATGGAGATCGACGATGAGTTAGTATGGATCCATCCAGACAGTT GCTTGAAGGGAAGAAAGGGATTCAGTACTACAAAAGGAAAATTGTATTCG TACTTCGAAGGCACCAAATTCCATCAAGCAGCAAAAGACATGGCGGAGAT AAAGGTCCTGTTCCCTAATGACCAGGAAAGTAATGAACAACTGTGTGCCT ACATATTGGGTGAGACCATGGAAGCAATCCGCGAAAAGTGCCCGGTCGAC CATAACCCGTCGTCTAGCCCGCCCAAAACGTTGCCGTGCCTTTGCATGTA TGCCATGACGCCAGAAAGGGTCCACAGACTTAGAAGCAATAACGTCAAAG AAGTTACAGTATGCTCCTCCACCCCCCTTCCTAAGCACAAAATTAAGAAT GTTCAGAAGGTTCAGTGCACGAAAGTAGTCCTGTTTAATCCGCACACTCC CGCATTCGTTCCCGCCCGTAAGTACATAGAAGTGCCAGAACAGCCTACCG CTCCTCCTGCACAGGCCGAGGAGGCCCCCGAAGTTGTAGCGACACCGTCA CCATCTACAGCTGATAACACCTCGCTTGATGTCACAGACATCTCACTGGA TATGGATGACAGTAGCGAAGGCTCACTTTTTTCGAGCTTTAGCGGATCGG ACAACTCTATTACTAGTATGGACAGTTGGTCGTCAGGACCTAGTTCACTA GAGATAGTAGACCGAAGGCAGGTGGTGGTGGCTGACGTTCATGCCGTCCA AGAGCCTGCCCCTATTCCACCGCCAAGGCTAAAGAAGATGGCCCGCCTGG CAGCGGCAAGAAAAGAGCCCACTCCACCGGCAAGCAATAGCTCTGAGTCC CTCCACCTCTCTTTTGGTGGGGTATCCATGTCCCTCGGATCAATTTTCGA CGGAGAGACGGCCCGCCAGGCAGCGGTACAACCCCTGGCAACAGGCCCCA CGGATGTGCCTATGTCTTTCGGATCGTTTTCCGACGGAGAGATTGATGAG CTGAGCCGCAGAGTAACTGAGTCCGAACCCGTCCTGTTTGGATCATTTGA ACCGGGCGAAGTGAACTCAATTATATCGTCCCGATCAGCCGTATCTTTTC CACTACGCAAGCAGAGACGTAGACGCAGGAGCAGGAGGACTGAATACTGA CTAACCGGGGTAGGTGGGTACATATTTTCGACGGACACAGGCCCTGGGCA CTTGCAAAAGAAGTCCGTTCTGCAGAACCAGCTTACAGAACCGACCTTGG AGCGCAATGTCCTGGAAAGAATTCATGCCCCGGTGCTCGACACGTCGAAA GAGGAACAACTCAAACTCAGGTACCAGATGATGCCCACCGAAGCCAACAA AAGTAGGTACCAGTCTCGTAAAGTAGAAAATCAGAAAGCCATAACCACTG AGCGACTACTGTCAGGACTACGACTGTATAACTCTGCCACAGATCAGCCA GAATGCTATAAGATCACCTATCCGAAACCATTGTACTCCAGTAGCGTACC GGCGAACTACTCCGATCCACAGTTCGCTGTAGCTGTCTGTAACAACTATC TGCATGAGAACTATCCGACAGTAGCATCTTATCAGATTACTGACGAGTAC GATGCTTACTTGGATATGGTAGACGGGACAGTCGCCTGCCTGGATACTGC AACCTTCTGCCCCGCTAAGCTTAGAAGTTACCCGAAAAAACATGAGTATA GAGCCCCGAATATCCGCAGTGCGGTTCCATCAGCGATGCAGAACACGCTA CAAAATGTGCTCATTGCCGCAACTAAAAGAAATTGCAACGTCACGCAGAT GCGTGAACTGCCAACACTGGACTCAGCGACATTCAATGTCGAATGCTTTC GAAAATATGCATGTAATGACGAGTATTGGGAGGAGTTCGCTCGGAAGCCA ATTAGGATTACCACTGAGTTTGTCACCGCATATGTAGCTAGACTGAAAGG CCCTAAGGCCGCCGCACTATTTGCAAAGACGTATAATTTGGTCCCATTGC AAGAAGTGCCTATGGATAGATTCGTCATGGACATGAAAAGAGACGTGAAA GTTACACCAGGCACGAAACACACAGAAGAAAGACCGAAAGTACAAGTGAT ACAAGCCGCAGAACCCCTGGCGACTGCTTACTTATGCGGGATTCACCGGG AATTAGTGCGTAGGCTTACGGCCGTCTTGCTTCCAAACATTCACACGCTT TTTGACATGTCGGCGGAGGATTTTGATGCAATCATAGCAGAACACTTCAA GCAAGGCGACCCGGTACTGGAGACGGATATCGCATCATTCGACAAAAGCC AAGACGACGCTATGGCGTTAACCGGTCTGATGATCTTGGAGGACCTGGGT GTGGATCAACCACTAGTCGACTTGATCGAGTGCGCCTTTGGAGAAATATC ATCCACCCATCTACCTACGGGTACTCGTTTTAAATTCGGGGCGATGATGA AATCCGGAATGTTCCTCACACTTTTTGTCAACACAGTTTTGAATGTCGTT ATCGCCAGCAGAGTACTAGAAGAGCGGCTTAAAACGTCCAGATGTGCAGC GTTCATTGGCGACGACAACATCATACATGGAGTAGTATCTGACAAAGAAA TGGCTGAGAGGTGCGCCACCTGGCTCAACATGGAGGTTAAGATCATCGAC GCAGTCATCGGTGAGAGACCACCTTACTTCTGCGGCGGATTTATCTTGCA AGATTCGGTTACTTCCACAGCGTGCCGCGTGGCGGACCCCCTGAAAAGGC TGTTTAAGTTGGGTAAACCGCTCCCAGCCGACGACGAGCAAGACGAAGAC AGAAGACGCGCTCTGCTAGATGAAACAAAGGCGTGGTTTAGAGTAGGTAT AACAGGCACTTTAGCAGTGGCCGTGACGACCCGGTATGAGGTAGACAATA TTACACCTGTCCTACTGGCATTGAGAACTTTTGCCCAGAGCAAAAGAGCA TTCCAAGCCATCAGAGGGGAAATAAAGCATCTGTACGGTGGTCCTAAATA GTCAGCATAGTACATTTCATCTGACTAATACTACAACACCACCACCTCTA GACCATGGATCCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCG TTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGT AATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCT GAATGGCGAATGGCGCTTTGCCTGGTTTCCGGCACCAGAAGCGGTGCCGG AAAGCTGGCTGGAGTGCGATCTTCCTGAGGCCGATACTGTCGTCGTCCCC TCAAACTGGCAGATGCACGGTTACGATGGGCCCATCTACACCAACGTGAC CTATCCCATTACGGTCAATCCGCCGTTTGTTCCCACGGAGAATCCGACGG GTTGTTACTCGCTCACATTTAATGTTGATGAAAGCTGGCTACAGGAAGGC CAGACGCGAATTATTTTTGATGGCGTTAACTCGGCGTTTCATCTGTGGTG CAACGGGCGCTGGGTCGGTTACGGCCAGGACAGTCGTTTGCCGTCTGAAT TTGACCTGAGCGCATTTTTACGCGCCGGAGAAAACCGCCTCGCGGTGATG GTGCTGCGCTGGAGTGACGGCAGTTATCTGGAAGATCAGGATATGTGGCG GATGAGCGGCATTTTCCGTGACGTCTCGTTGCTGCATAAACCGACTACAC AAATCAGCGATTTCCATGTTGCCACTCGCTTTAATGATGATTTCAGCCGC GCTGTACTGGAGGCTGAAGTTCAGATGTGCGGCGAGTTGCGTGACTACCT ACGGGTAACAGTTTCTTTATGGCAGGGTGAAACGCAGGTCGCCAGCGGCA CCGCGCCTTTCGGCGGTGAAATTATCGATGAGCGTGGTGGTTATGCCGAT CGCGTCACACTACGTCTGAACGTCGAAAACCCGAAACTGTGGAGCGCCGA AATCCCGAATCTCTATCGTGCGGTGGTTGAACTGCACACCGCCGACGGCA CGCTGATTGAAGCAGAAGCCTGCGATGTCGGTTTCCGCGAGGTGCGGATT GAAAATGGTCTGCTGCTGCTGAACGGCAAGCCGTTGCTGATTCGAGGCGT TAACCGTCACGAGCATCATCCTCTGCATGGTCAGGTCATGGATGAGCAGA CGATGGTGCAGGATATCCTGCTGATGAAGCAGAACAACTTTAACGCCGTG CGCTGTTCGCATTATCCGAACCATCCGCTGTGGTACACGCTGTGCGACCG CTACGGCCTGTATGTGGTGGATGAAGCCAATATTGAAACCCACGGCATGG TGCCAATGAATCGTCTGACCGATGATCCGCGCTGGCTACCGGCGATGAGC GAACGCGTAACGCGAATGGTGCAGCGCGATCGTAATCACCCGAGTGTGAT CATCTGGTCGCTGGGGAATGAATCAGGCCACGGCGCTAATCACGACGCGC TGTATCGCTGGATCAAATCTGTCGATCCTTCCCGCCCGGTGCAGTATGAA GGCGGCGGAGCCGACACCACGGCCACCGATATTATTTGCCCGATGTACGC GCGCGTGGATGAAGACCAGCCCTTCCCGGCTGTGCCGAAATGGTCCATCA AAAAATGGCTTTCGCTACCTGGAGAGACGCGCCCGCTGATCCTTTGCGAA TACGCCCACGCGATGGGTAACAGTCTTGGCGGTTTCGCTAAATACTGGCA GGCGTTTCGTCAGTATCCCCGTTTACAGGGCGGCTTCGTCTGGGACTGGG TGGATCAGTCGCTGATTAAATATGATGAAAACGGCAACCCGTGGTCGGCT TACGGCGGTGATTTTGGCGATACGCCGAACGATCGCCAGTTCTGTATGAA CGGTCTGGTCTTTGCCGACCGCACGCCGCATCCAGCGCTGACGGAAGCAA AACACCAGCAGCAGTTTTTCCAGTTCCGTTTATCCGGGCAAACCATCGAA GTGACCAGCGAATACCTGTTCCGTCATAGCGATAACGAGCTCCTGCACTG GATGGTGGCGCTGGATGGTAAGCCGCTGGCAAGCGGTGAAGTGCCTCTGG ATGTCGCTCCACAAGGTAAACAGTTGATTGAACTGCCTGAACTACCGCAG CCGGAGAGCGCCGGGCAACTCTGGCTCACAGTACGCGTAGTGCAACCGAA CGCGACCGCATGGTCAGAAGCCGGGCACATCAGCGCCTGGCAGCAGTGGC GTCTGGCGGAAAACCTCAGTGTGACGCTCCCCGCCGCGTCCCACGCCATC CCGCATCTGACCACCAGCGAAATGGATTTTTGCATCGAGCTGGGTAATAA GCGTTGGCAATTTAACCGCCAGTCAGGCTTTCTTTCACAGATGTGGATTG GCGATAAAAAACAACTGCTGACGCCGCTGCGCGATCAGTTCACCCGTGCA CCGCTGGATAACGACATTGGCGTAAGTGAAGCGACCCGCATTGACCCTAA CGCCTGGGTCGAACGCTGGAAGGCGGCGGGCCATTACCAGGCCGAAGCAG CGTTGTTGCAGTGCACGGCAGATACACTTGCTGATGCGGTGCTGATTACG ACCGCTCACGCGTGGCAGCATCAGGGGAAAACCTTATTTATCAGCCGGAA AACCTACCGGATTGATGGTAGTGGTCAAATGGCGATTACCGTTGATGTTG AAGTGGCGAGCGATACACCGCATCCGGCGCGGATTGGCCTGAACTGCGAG CTGGCGCAGGTAGCAGAGCGGGTAAACTGGCTCGGATTAGGGCCGCAAGA AAACTATCCCGACCGCCTTACTGCCGCCTGTTTTGACCGCTGGGATCTGC CATTGTCAGACATGTATACCCCGTACGTCTTCCCGAGCGAAAACGGTCTG CGCTGCGGGACGCGCGAATTGAATTATGGCCCACACCAGTGGCGCGGCGA CTTCCAGTTCAACATCAGCCGCTACAGTCAACAGCAACTGATGGAAACCA GCCATCGCCATCTGCTGCACGCGGAAGAAGGCACATGGCTGAATATCGAC GGTTTCCATATGGGGATTGGTGGCGACGACTCCTGGAGCCCGTCAGTATC GGCGGAATTCCAGCTGAGCGCCGGTCGCTACCATTACCAGTTGGTCTGGT GTCAAAAATAATAATAACCGGGCAGGGGGGATCCTAGACGCTACGCCCCA ATGATCCGACCAGCAAAACTCGATGTACTTCCGAGGAACTGATGTGCATA ATGCAGGAATTCGATATCAAGCTAGATCTCACGTGAGCATGCGTTTAAAC TGGGCCCAATGTTCCCCAATGATCCGACCAGCAAAACTCGATGTACTTCC GAGGAACTGATGTGCATAATGCATCAGGCTGGTACATTAGATCCCCGCTT ACCGCGGGCAATATAGCAACACTAAAAACTCGATGTACTTCCGAGGAAGC GCAGTGCATAATGCTGCGCAGTGTTGCCACATAACCACTATATTAACCAT TTATCTAGCGGACGCCAAAAACTCAATGTATTTCTGAGGAAGCGTGGTGC ATAATGCCACGCAGCGTCTGCATAACTTTTATTATTTCTTTTATTAATCA ACAAAATTTTGTTTTTAACATTTCAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAATTTAAATTAATTAAGCGGCCGCCTCGAGGACGTCAGGT AGGCCTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTA AATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGC TTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTC GCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCC AGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATGAGTTGGGTGCACGAG TGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTT CGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATG TGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCC GCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAA AAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCAT AACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAG GACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACT CGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGA GCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTAT TAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGG ATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGC TGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCG GTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTT ATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGAT CGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAG TTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAA AGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCGCTTA ACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAG GATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACA AAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACC AACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATA CTGGTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTA GCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGC CAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTAC CGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCC AGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCT ATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGG TAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGA AACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGA GCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACG CCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCT CACATGTGGGAGGCCTGAGTACTTAATACGACTCACTATAGG

TABLE 3 T7StuIR-LacZ (13.092 bp) components Nt → nt Part from: Description 1_7646 SV 1_7646 5′ end untranscribed + replicase (nsp1, nsp2, nsp3 and nsp4) + subgenomic promoter 7647_7654 Oligos Multi cloning site MCS 7,655_10,711 Plasmid LacZ gene (coding for b-galactosidase) 10712_10864 Oligos Multi cloning site MCS 10864_11174 SV 11,393_11,703 3′ end untranslated region Oligos Multi cloning site MCS 11,236 Oligos XhoI Restriction site for linearization 11,254 Oligos StuI new restriction site for subcloning 11248_13051 Bacterial part pUC 11,377_12,237 plasmid Beta Lactamase (=Amp resistant gene) 12,963_12,999 plasmid Bacterial DNA replication origin ColE1 13051_13092 Oligos T7 promoter 13064 oligos StuI new restriction site for subcloning 13074_13092 oligos T7 core sequence

Paper Example 1 Production of C6/36 Packaging Cell Line with Rederived Ar-339 Plasmids

Plasmids SP6-H and SP6-R can be used to engineer insect plasmids to generate a mosquito C6/36-derived packaging cell line producing Ar-339 Sindbis vectors. Three plasmids with the Opal2 mosquito promoter (described in Theilmann et al., J. Virology, 1995, 69(12):7775-7781) are required to constitutively express viral sequences in mosquito cells. The replicon plasmid (pIZ-Ar339-R) will have the replicase, subgenomic promoter and gene of interest and the other two will contain the split helper sequences, one with capsid protein only (PIB-Ar339-C) and the second one with E1, E2, E3 and K6 envelope proteins (pIZ-Ar339-H). In a first step the C6/36 cell line (available from the American Type Culture Collection, ATCC, Manassas, Va. as ATCC CRL 1660) will be transfected with replicon plasmid (pIZ-Ar339-R) and clones will be selected. In a second step the previous clones containing the replicon will be transfected with the capsid plasmid (PIB-Ar339-C) and replicon and capsid positive clones selected. In the last step, the 2^(nd) helper plasmid (pIZ-Ar339-H) will be transfected into the previously isolated clones to generate the packaging cell line that express all three plasmids.

1.—Cloning of SP6-R Replicase into PIZ/V5-His Plasmid

In order to clone the Ar-339 replicase in PIZ/V5-His (Invitrogen, San Diego, Calif.) it is necessary to introduce a SacI RE site before the SP6 promoter.

1.1—PCR Reactions will be performed on SP6-R#406 plasmid using primers SacI_SP6F/cDNA-1R, and then the 2340 by band will be cloned in pcr4blunt_topo vector (Invitrogen, San Diego, Calif.). The new plasmids TOPO-Rep1

Primer sequences: SacI_SP6F GGCTAGAGCTCATTTAGGTGACA (SacI) cDNA-1R GTAACAAGATCTCGTGCCGTGACA

1.2.—The SP6-R#406 plasmid will be digested with BglII/NotI and cloned the 5776 bp band into BglII/NotI TOPO-Rep1 to make new plasmid TOPO-Ar339-R

1.3.—The 8085 by SacI/NotI TOPO-Ar339-R band will be cloned into SacI/NotI PIZ/V5-His to generate pIZ-Ar339-R.

2.—Cloning of Ar-339 Helper P

In order to minimize the presence of recombinant replicative competent virus, the helper genome will be split into two plasmids: PIB-Ar339-C and pIZ-Ar339-H.

2.1 Construction of pIB-Ar339-C

The Ar-339 Capsid DNA sequence will be cloned into pIB-V5-His (Invitrogen, San Diego, Calif.) at the BamHI/SpeI site of the vector. PCR will be performed on plasmid SP6-H#432 using primers C1-F and C1-R to obtain the Ar-339 capsid 1133 bp DNA band.

C1-F GGA TCT CCG GAT CCC CTG AAA AGG (BamHI) C1-R GTG ACC AGT GGA CTA GTG GAC CAC TCT TC (SpeI)

The band will be digested and cloned into the pIB-V5-His vector at the BamHI/SpeI site to make the new plasmid pIB-Ar-339-C.

2.2 Construction of pIZ-Ar339-H

The first step is to clone the 5′ end of Ar-339 (from nt 425 to 692) into pIZ/V5-His. PCR reactions will be performed on SP6H#432 plasmid with primers 416B-F and 676NB-R. Restriction sites will be included in this no coding sequence to allow further cloning of helper sequence.

416B-F GGA TCT CCG GAT CCC CTG AAA AGG CTG T (BamHI) 676NB-R GAT GAA AGG ATC C TC GCG AAC TAT TTA GGA CCA CCG (BamHI/NruI)

The 296 by band will be digested with BamHI and cloned in the BamHI site of pIZ/V5-His to make pIZ-5END plasmid.

In a second step SP6-H#432 plasmid will be digested with NruI and XhoI and the 3435 by band will be cloned into the NruI/XhoI site of the pIZ-5END plasmid.

REFERENCES

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The present invention is not to be limited in scope by the specific embodiments described herein. Indeed, various modifications of the invention in addition to those described herein will become apparent to those skilled in the art from the foregoing description and the accompanying figures. Such modifications are intended to fall within the scope of the appended claims.

It is further to be understood that all values are approximate, and are provided for description.

Patents, patent applications, publications, product descriptions, and protocols are cited throughout this application, the disclosures of which are incorporated herein by reference in their entireties for all purposes.

APPENDIX A

TABLE I Primers used for Sindbis cDNA cloning. PRIMER SEQUENCE (5′→3′) SV NT cDNA bp cDNA-1F ATTGACGGCGTAGTACAC (SEQ ID NO: 7)     1-20 cDNA-1 cDNA-1R GTAACA AGATCT CGTGCCGTGACA (Bgl II) (SEQ ID NO: 8)  2299-2276 2276 bp cDNA-2F GGCACG AGATCT TGTTACCAGC (Bgl II) (SEQ ID NO: 9)  2281-2303 cDNA-2 cDNA-2R CTTTCTTT CCTAGG CACACAGTCATTCTT (Avr II) (SEQ ID NO: 10)  4265-4293 2012 bp cDNA-3F GACTGTGTG CCTAGG AAAGAAAGTG (Avr II) (SEQ ID NO: 11)  4271-4295 cDNA-3A SV-6932R CACACCCAGGTCCTCCAAGATC (SEQ ID NO: 12)  6932-6953 2682 bp SV-6882F GCATCATTCGACAAAAGCCAAG (SEQ ID NO: 13)  6882-6903 cDNA-3B SV-C3R CTCT TCTAGA GGTGGTGGTGTTGTAGTATT (XbaI) (SEQ ID NO: 14)  7626-7656 774 bp cDNA-4F GGATCC CCTGAAAAGGCTGTTTAAG (BamHI) (SEQ ID NO: 15)  7334-7359 cDNA-4 cDNA-4R TCATGTC TGATCA AGTCCGGTGA (BclI) (SEQ ID NO: 16)  9370-9348 2014 bp cDNA-5F GGACT TGATCA GACATGACGACCA (BclI) (SEQ ID NO: 17)  9353-9376 CDNA-5 cDNA-5R TTTTTGAAATGTTAAAAACAAAATTTTGTTG (SEQ ID NO: 18) 11678-11703 2350 bp The restriction endonuclease recognition sites are underlined. Sindbis virus nucleotide numbers follow Strauss et al (1984) sequence (Accession# NC_001547.1)

TABLE II Primers used to generate vector polylinkers. PRIMER (RE) SEQUENCE 5′→3′ Poly1-T7(+) CCCACATGTGGGAGGCTAGAGTACTTAATACGACTCACTATAGGATTGACGGCGTAGTACACACTATTGAATCAAACAGCCGACC (AflIII) (SEQ ID NO: 19) Poly1- CCCACATGTGGGAGGCTAGAGTACATTTAGGTGACACTATAGAAATTGACGGCGTAGTACACACTATTGAATCAAACAGCCGACC SP6(+) (SEQ ID NO: 20) (AflIII) Poly-2(−) GGCGCGCCTCTAGACTAGCCTAGGTATGGAAGATCTTCCGCGGATCCGCCTAGTGCAATTGGTCGGCTGTTTGATTCAAT (XbaI) (SEQ ID NO: 21) Poly-3(+) AGGCTAGTCTAGAGGCGCGCCGATCTCACGTGAGCATGCGTTTAAACTGGGCCCAATGTTAACATTTCAAAAAAAAAAAAAAAAA XbaI) (SEQ ID NO: 22) Poly-4(−) GGTGATGACGTCCTCGAGGCGGCCGCTTAATTAATTTAAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAAATGT (AatII) (SEQ ID NO: 23) PCRPoly-1F ATATATATCCCACATGT (AflIII) (SEQ ID NO: 24) PCRPoly-2R GCGCGCCTCTAGA (SEQ ID NO: 25) PCRPoly-3F AGGCTAGTCTAGAGGC (SEQ ID NO: 26) PCRPoly-4R GGTGATGACGTCCT (AatII) (SEQ ID NO: 27) Restriction endonuclease recognition sites (RE) are underlined. Overlapping sequences are shown in bold.

TABLE III PRIMERS USED IN SITE-DIRECTED MUTAGENESIS OF E2 GENE. PRIMER SEQUENCE 5′→3′ RE E2-I3-F GCAAAAGAAGCGTCA TC GA T GACTTTACCCTGACCAGC ClaI (SEQ ID NO: 28) E2-I3-R GCTGGTCAGGGTAAAGTC A TC GA TGACGCTTCTTTTGC ClaI (SEQ ID NO: 29) E2-K70-F CTACATGTCGCTT A AGCAGGATCACACCGTTAAAG Afl II (SEQ ID NO: 30) E2-K70-R CTTTAACGGTGTGATCCTGCT T AAGCGACATGTAG Afl II (SEQ ID NO: 31) E2-E181-F CGGG C CCGCACGCTTATACATCCTACCTG G AAGAATCATC Sma I (SEQ ID NO: 32) E2-E181-R GATGATTCTT C CAGGTAGGATGTATAAGCGTGCGG G CCCG Sma I (SEQ ID NO: 33) E2-V251-F GACTTGATC C GACATGACGACCACACGG T CCAAGGG Mme I (SEQ ID NO: 34) E2-V251-R CCCTTGG A CCGTGTGGTCGTCATGTC G GATCAAGTC Mme I (SEQ ID NO: 35) Nucleotides changed are underlined and the new restriction sites generated are indicated (RE)

TABLE IV Nucleotide differences between Ar-339, JT vectors and Sindbis virus Strauss sequence (Strauss et al. 1984)

Nucleotide numbers follow the Strauss et al sequence. (Acession #NC_001547.1)

TABLE V Nucleotide differences between JT vectors and Strauss sequence

Strauss et al 1984 sequence. (Accession #NC_001547.1)

TABLE VI Summary of amino acid differences between JT and Ar-339 vectors

TABLE VII TITER OF CHIMERIC VIRAL VECTORS. BHK-21 cells ES-2/Fluc cells Mosec cells VIRAL VECTOR (TU/mL) (TU/mL) (TU/mL) JT-BB/SP6-ARepLacZ 3 × 10⁶ 3 × 10⁴ 3 × 10⁴ SP6-AH/JT-RepLacZ 3 × 10⁴ 3 × 10³ 3 × 10³ SP6-AH/SP6-ARepLacZ 3 × 10⁴ 3 × 10³ 3 × 10³ JT-BB/JT-RepLacZ 3 × 10⁶ 3 × 10⁴ 3 × 10⁴ Vectors were titered in BHK-21, ES-2/Fluc and Mosec cell lines. TU transducing units

TABLE VIII TITERS OF E2 MUTANT VECTORS. BHK-21 ES-2/Fluc Mosec VIRAL VECTOR (TU/mL) (TU/mL) (TU/mL) A (JT-BB/SP6-RFluc) 10⁷ 10⁵ 10⁶ C (SP6-H/SP6-RFluc) 10⁵ 10³ 10⁴ Mut-1 (SP6H-K70/SP6-RFluc) 10⁴ 10³ 10⁵ Mut-2 (SP6H-K70-V251/SP6- 10⁵ 10⁴ 10² RFluc) Mut-4 (SP6H-I3-K70-E181-V251/ 10⁶ 10⁴ 10⁵ SP6-RFluc) Vectors were titered in BHK-21, ES-2/Fluc and Mosec cell lines. TU transducing units 

1. An isolated nucleic acid comprising the nucleotide sequence encoding the DM Helper plasmid T7-DM-H101 as set forth in SEQ ID NO:
 60. 2. A method for producing defective Sindbis viral vectors comprising the steps of (a) providing a linearized Replicon plasmid comprising the nucleotide sequence as set forth in SEQ ID NO: 65 and a linearized Helper plasmid T7-DM-H101 comprising the nucleotide sequence encoding said Helper plasmid as set forth in SEQ ID NO: 60; (b) transcribing said Replicon plasmid and said Helper plasmid to produce RNA; (c) collecting the RNA transcribed in step (b) and transfecting cells with said RNA; (d) incubating said transfected cells for a time and at a temperature effective for producing defective Sindbis viral vectors; and (e) collecting said defective Sindbis viral vectors from the medium of said transfected cells.
 3. A defective Sindbis virus vector produced by the method of claim
 2. 4. A method for treating a mammal suffering from a tumor which expresses higher levels of HALR than normal cells of the same lineage comprising systemically administering to a mammal in need of such treatment an amount effective to treat said mammal of the defective Sindbis virus vector of claim
 3. 5. A pharmaceutical formulation for treating a mammal suffering from a tumor which expresses higher levels of HALR than normal cells of the same lineage comprising the defective Sindbis virus vector of claim 3 and a pharmaceutically acceptable carrier or diluent.
 6. An isolated nucleic acid comprising the nucleotide sequence encoding plasmid T7-StuI-RLac7#202 as set forth in SEQ ID NO:
 65. 